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Specifications:
| Application | Cell culture, molecular biology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Enzymes and Substrates | Forms | Solid |
| Product Brand | MedChem Express | ||
| Product Grade | Molecular Biology | ||
DNase I from bovine pancreas is a highly active endonuclease enzyme that catalyzes the hydrolytic cleavage of DNA into oligonucleotides. Classified under EC 3.1.21.1, DNase I plays an important biological role in degrading extracellular DNA, thereby helping regulate inflammatory responses and maintain cellular homeostasis.
This enzyme is widely used in molecular biology, protein purification, and cell biology laboratories to remove contaminating DNA from RNA or protein samples. DNase I is particularly valuable in protein extraction workflows, where it reduces viscosity caused by genomic DNA and improves sample handling and purification efficiency.
DNase I activity is dependent on divalent metal ions, particularly Ca²⁺ and Mg²⁺, which stabilize the enzyme and enable catalytic activity. In the presence of Mg²⁺, DNase I randomly cleaves phosphodiester bonds within single DNA strands, while Mn²⁺ ions promote double-stranded cleavage at similar sites. The enzyme exhibits optimal activity at pH 7–8 and has an approximate molecular weight of 31 kDa.
Key Features
- Endonuclease enzyme that degrades DNA molecules
- Derived from bovine pancreas
- High enzymatic activity (>2000 Kunitz units/mg)
- Effective for removal of genomic DNA contamination
- Reduces viscosity during protein extraction and cell lysis
- Compatible with many molecular biology and biochemical protocols
- Soluble enzyme suitable for laboratory research applications
Applications
- Removal of DNA contamination during RNA or protein purification
- Reduction of viscosity in cell lysates during protein extraction
- DNA degradation in molecular biology experiments
- Cell and tissue lysate preparation
- Enzymatic digestion of extracellular DNA
- Biochemical and enzymology research
Available Sizes
| Catalog Number | Size |
|---|---|
| HY-108882-50MG | 50 mg |
| HY-108882-100MG | 100 mg |
| HY-108882-250MG | 250 mg |
| HY-108882-500MG | 500 mg |
Technical Specifications
| Parameter | Specification |
|---|---|
| Enzyme Name | DNase I |
| Synonyms | DNase, Deoxyribonuclease I |
| EC Number | 3.1.21.1 |
| CAS Number | 9003-98-9 |
| Biological Source | Bovine pancreas |
| Molecular Weight | ~31 kDa |
| Appearance | Off-white to light yellow solid |
| Optimal pH | 7–8 |
| Enzyme Activity | >2000 Kunitz U/mg |
| Solubility | ≥4 mg/mL in water |
| Activators | Ca²⁺, Mg²⁺, Mn²⁺, Co²⁺, Zn²⁺ |
| Inhibitors | SDS, DTT, β-mercaptoethanol, chelating agents |
Enzyme Activity and Stability
DNase I activity depends on the presence of divalent metal ions. Ca²⁺ stabilizes the enzyme, while Mg²⁺ promotes random cleavage of DNA strands. The enzyme remains stable below 65°C, and its activity can be inactivated by heating at 65°C for 10 minutes or by phenol–chloroform extraction.
Chelating agents such as EDTA should be avoided in reaction buffers because they bind the metal ions required for enzymatic activity.
Storage and Handling
- Store under recommended conditions indicated in the Certificate of Analysis
- Prepare enzyme solutions freshly when possible
- Avoid repeated freeze–thaw cycles by storing in aliquots
- Use buffers free of metal chelators such as EDTA
MCE DNase I (Bovine Pancreas) is a powerful DNA-degrading enzyme widely used in molecular biology and biochemical workflows. Its ability to efficiently digest DNA makes it an essential reagent for RNA purification, protein extraction, and nucleic acid research, providing reliable results in laboratory applications requiring removal of DNA contamination.
- Pack Size: 50 mg 100 mg 500 mg 250 mg