Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL), 1000 units

Thermo Scientific™ DNase I, RNase-free, High Concentration (HC) is a recombinant endonuclease that degrades both single-stranded (ssDNA) and double-stranded DNA (dsDNA). The enzyme hydrolyzes phosphodiester linkages, yielding mono- and oligo-deoxyribonucleotides with 5′-phosphate and 3′-OH termini.

Activity is Ca²⁺-dependent and enhanced by Mg²⁺ or Mn²⁺. In Mg²⁺ conditions, DNase I cleaves dsDNA strands independently in a random fashion, while in Mn²⁺ conditions it cleaves both strands at similar positions, generating blunt-ended or nearly blunt fragments.

Supplied at 50 U/µL (1,000 units total), this high-concentration format is optimized for RNA workflows where DNA contamination must be completely removed.

Key Features

• Recombinant enzyme – produced from non-animal host; low intrinsic RNase contamination.
• High concentration – 50 U/µL format for flexibility in sensitive applications.
• Versatile activity – cleaves ssDNA and dsDNA into short oligonucleotides.
• Metal ion control – cleavage specificity influenced by Mg²⁺ (random cuts) vs Mn²⁺ (near-blunt cuts).
• High purity – DNase I validated as RNase-free for reliable RNA applications.

Applications

• Preparation of DNA-free RNA samples.
• Removal of DNA template following in vitro transcription.
• Elimination of contaminating DNA before RT-PCR and RT-qPCR.
• Nick translation for DNA labeling when combined with DNA Polymerase I.
• DNase I footprinting for DNA-protein interaction studies.
• Generation of random DNA fragment libraries under Mn²⁺ conditions.

Technical Specifications

Thermo Scientific™ DNase I, RNase-free, HC (50 U/µL) provides a reliable, high-purity enzyme for applications requiring the complete removal of DNA from RNA samples. With its recombinant production, high concentration, and compatibility with molecular biology workflows, it is the enzyme of choice for preparing RNA suitable for RT-PCR, RT-qPCR, transcriptomics, and RNA sequencing workflows.