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Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
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Specifications:
| Application | Reverse Transcription | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit is designed for the generation of first-strand cDNA for two-step RT-qPCR applications. The kit provides an optimized reverse transcription system for converting RNA into high-quality cDNA suitable for downstream real-time PCR analysis.
The kit is supplied in a convenient two-tube format, consisting of a 5X VILO Reaction Mix and a 10X SuperScript™ III Enzyme Blend. The enzyme blend contains SuperScript™ III Reverse Transcriptase, a genetically engineered MMLV reverse transcriptase with reduced RNase H activity and improved thermostability, enabling efficient cDNA synthesis across a wide range of RNA input amounts.
This kit is suitable for research laboratories performing gene expression analysis, RNA quantification workflows, and other two-step RT-qPCR applications.
Key Features
- Designed for first-strand cDNA synthesis
- Suitable for two-step RT-qPCR workflows
- Uses SuperScript™ III Reverse Transcriptase
- Reduced RNase H activity for improved cDNA synthesis efficiency
- Improved thermostability for reliable reverse transcription performance
- Optimized for a wide range of RNA input amounts
- Supports synthesis of cDNA up to 10 kb
- Convenient master mix reaction format
- Available in 50-reaction and 250-reaction formats
- For Research Use Only
Ordering Information
| Catalog Number | Number of Reactions | Unit Size |
|---|---|---|
| 11754050 | 50 reactions | Each |
| 11754250 | 250 reactions | Each |
Technical Specifications
| Parameter | Details |
|---|---|
| Product Name | SuperScript™ VILO™ cDNA Synthesis Kit |
| Brand | Invitrogen™ |
| Final Product Type | First-strand cDNA |
| Format | Kit |
| Reaction Format | Master Mix |
| Reagent Type | Reverse Transcription |
| Reverse Transcriptase | SuperScript™ III |
| Ribonuclease H Activity | Reduced |
| Starting Material | RNA |
| Technique | Reverse Transcription |
| Detection Method | qPCR |
| Application | Real-Time PCR / RT-qPCR |
| Optimal Reaction Temperature | 42°C |
| Reaction Speed | 60 minutes |
| Size of Final Product | Up to 10 kb |
| GC-Rich PCR Performance | High |
| Shipping Condition | Dry Ice |
| Storage Conditions | –5°C to –30°C |
| Use Statement | For Research Use Only. Not for diagnostic procedures. |
Kit Contents
| Component | Volume |
|---|---|
| 5X VILO Reaction Mix | 200 µL |
| 10X SuperScript™ III Enzyme Blend | 100 µL |
Reagents are provided for 50 or 250 20-µL cDNA synthesis reactions, depending on the selected kit format.
Applications
| Application Area | Typical Use |
|---|---|
| Two-Step RT-qPCR | First-strand cDNA synthesis prior to qPCR amplification |
| Gene Expression Analysis | Conversion of RNA into cDNA for expression profiling |
| RNA Quantification Workflows | Preparation of cDNA from RNA templates |
| Molecular Biology Research | Reverse transcription for downstream PCR-based analysis |
| GC-Rich Template Studies | cDNA synthesis where improved thermostability is beneficial |
| Research Laboratory Workflows | Routine and advanced reverse transcription applications |
Workflow Summary
The SuperScript™ VILO™ cDNA Synthesis Kit is used to convert RNA into first-strand cDNA in a reverse transcription reaction. The generated cDNA can then be used as template material in downstream real-time PCR / qPCR assays.
Typical workflow:
- Prepare RNA sample
- Set up reverse transcription reaction using VILO Reaction Mix and SuperScript™ III Enzyme Blend
- Incubate at the recommended reaction temperature
- Use the synthesized cDNA in downstream qPCR analysis
Storage and Handling Notes
The kit should be stored at –5°C to –30°C upon receipt. It is shipped on dry ice to maintain reagent stability during transport.
Recommended handling considerations include:
- Keep reagents frozen when not in use
- Thaw components on ice before reaction setup
- Mix gently and briefly centrifuge before use
- Avoid repeated freeze-thaw cycles where possible
- Use nuclease-free tubes, tips, and reagents
- Follow good molecular biology laboratory practices
The Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit is a reliable reverse transcription kit for generating first-strand cDNA in two-step RT-qPCR workflows. Powered by SuperScript™ III Reverse Transcriptase, the kit offers efficient cDNA synthesis, reduced RNase H activity, improved thermostability, and compatibility with a broad range of RNA inputs, making it suitable for gene expression and molecular biology research applications.
- Reactions: 50 rxn 250 rxn