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Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
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Specifications:
| Application | Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit combines reverse transcription and PCR amplification in a single tube, streamlining workflows for gene expression analysis, pathogen detection, and infectious disease surveillance. This validated and widely trusted kit is recognized by global public health organizations and has been a preferred choice of the US CDC for infectious disease monitoring.
With its optimized enzyme blend of SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase (hot-start), the kit delivers high specificity, sensitivity, and reproducibility across diverse real-time PCR platforms.
Key Features
- One-Step Format – Combines cDNA synthesis and PCR amplification in a single tube.
- High Performance RT – SuperScript™ III enables higher temperature cDNA synthesis for resolving difficult RNA secondary structures.
- Hot-Start PCR – Platinum™ Taq DNA Polymerase ensures improved specificity and reduced non-specific amplification.
- Probe-Based Detection – Compatible with LUX fluorogenic primers and dual-labeled fluorogenic probes.
- Validated for Public Health Use – Trusted by the US CDC and validated for infectious disease surveillance worldwide.
- Broad Instrument Compatibility – Suitable for rotor-based and block-based qPCR platforms.
Applications
- Gene expression analysis
- Pathogen detection
- Virus surveillance and monitoring
- Molecular epidemiology studies
Technical Specifications
| Property | Details |
|---|---|
| Product Type | One-Step qRT-PCR Kit |
| Product Line | SuperScript™ / Platinum™ |
| Polymerase | Taq DNA Polymerase (hot-start) |
| Reverse Transcriptase | SuperScript™ III |
| Sample Type | RNA |
| Detection Method | Primer-probe |
| PCR Method | One-step RT-qPCR |
| Reaction Speed | Standard |
| Compatible Equipment | 7500 System, BioRad iCycler iQ/iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
| Shipping Condition | Dry Ice |
| Storage Conditions | –20 °C (ROX Reference Dye stored in the dark) |
| Unit Size | Each |
Kit Contents & Ordering Information
| Catalog Number | Reactions | Kit Contents |
|---|---|---|
| 11732020 | 100 reactions | • 100 μL SuperScript™ III/Platinum™ Taq Mix • 2 × 1.25 mL 2X Reaction Mix • 1 mL Magnesium Sulfate (50 mM) • 100 μL ROX Reference Dye (25 μM) |
| 11732088 | 500 reactions | • 500 μL SuperScript™ III/Platinum™ Taq Mix • 12.5 mL 2X Reaction Mix • 2 × 1 mL Magnesium Sulfate (50 mM) • 500 μL ROX Reference Dye (25 μM) |
Other configurations available:
- SuperScript™ III Platinum™ One-Step qRT-PCR Kit with ROX passive reference
- SuperScript™ III Platinum™ SYBR Green One-Step qRT-PCR Kit
- SuperScript™ III Platinum™ SYBR Green One-Step qRT-PCR Kit with ROX passive reference
The Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides a fast, sensitive, and validated solution for RNA detection and quantification. With its proven reverse transcriptase–polymerase blend, probe-based detection compatibility, and global track record in infectious disease surveillance, it is a trusted choice for both research and public health laboratories.
- Pack Size: 500 Reactions 100 Reactions