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Thermo Scientific™ ActivX™ TAMRA-FP Serine Hydrolase Probe, 6.8 μg
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Specifications:
| Application | Protein Assays & Analysis, Mass Spectrometry, Chemiluminescent Western Blot Imaging | ||
| Storage Temperature | –80°C | ||
| Product Type | Proteins & Peptides | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ ActivX™ TAMRA-FP Serine Hydrolase Probe is an activity-based fluorophosphonate probe designed for labeling and detecting enzymatically active serine hydrolases. The probe contains a reactive fluorophosphonate (FP) group that covalently labels the active-site serine residue of functional serine hydrolases, together with a TAMRA fluorescent tag for direct detection.
The TAMRA-FP probe is suitable for profiling serine hydrolase activity using fluorescent gel imaging, capillary electrophoresis, Western blot, chemiluminescence, and mass spectrometry workflows. An anti-TAMRA antibody can also be used for immuno-enrichment of TAMRA-FP-labeled proteins.
Key Features
| Feature | Details |
|---|---|
| Activity-Based Probe | Selectively labels enzymatically active serine hydrolases |
| Fluorophosphonate Reactive Group | Covalently targets the active-site serine residue |
| TAMRA Fluorescent Tag | Enables fluorescent detection of labeled proteins |
| Direct Activity Profiling | Suitable for fluorescent gel imaging and capillary electrophoresis |
| Immuno-Enrichment Compatible | Anti-TAMRA antibody can be used to enrich TAMRA-labeled proteins |
| Inhibitor Screening Support | Useful for assessing inhibitor specificity, affinity, and active-site binding |
| Multiple Workflow Compatibility | Supports fluorescent detection, Western blot, chemiluminescence, and mass spectrometry workflows |
Applications
ActivX™ TAMRA-FP Serine Hydrolase Probe is suitable for:
- Serine hydrolase activity profiling
- Detection of active serine hydrolases in cells, lysates, tissues, and recombinant proteins
- Fluorescent gel imaging of active hydrolases
- Capillary electrophoresis-based enzyme profiling
- Mass spectrometry-based serine hydrolase analysis
- Small molecule inhibitor screening
- Active-site inhibition studies
- Inhibitor target and off-target profiling
- Immuno-enrichment of TAMRA-labeled proteins using anti-TAMRA antibody
- Profiling proteases, lipases, esterases, amidases, and peptidases
Product Specifications
| Specification | Details |
|---|---|
| Product Name | ActivX™ TAMRA-FP Serine Hydrolase Probe |
| Catalog Number | 88318 |
| Product Line | ActivX™ |
| Type | Fluorophosphonate, FP probe |
| Target | Serine hydrolase |
| Substrate Type | Serine hydrolase substrate |
| Formulation | TAMRA-fluorophosphonate |
| Molecular Weight | 680 |
| Label / Dye | TAMRA fluorescent label |
| Quantity | 6.8 µg |
| Assay | Protein quantitation assay |
| Application | Pull-down |
| Format | Tube, spin column |
| Detection Method | Chemiluminescence, mass spectrometry, Western blot |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Workflow Overview
| Workflow | Description |
|---|---|
| Active-Site Labeling | FP group covalently labels active-site serine residues in active serine hydrolases |
| Fluorescent Detection | TAMRA tag enables direct visualization by fluorescent gel imaging |
| Capillary Electrophoresis | Supports activity profiling using capillary-based separation workflows |
| Immuno-Enrichment | Anti-TAMRA antibody can be used to enrich labeled proteins |
| Mass Spectrometry | Supports identification and profiling of active serine hydrolases and inhibitor targets |
| Inhibitor Profiling | Competitive labeling can be used to evaluate inhibitor binding and off-target activity |
Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| 88318 | ActivX™ TAMRA-FP Serine Hydrolase Probe | 6.8 µg |
Storage & Handling
Store the probe at -80 °C, protected from light. Stock solutions prepared in DMSO can be stored at -20 °C for up to 6 months. Handle using appropriate laboratory personal protective equipment and avoid repeated freeze-thaw cycles where possible.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures. This product is subject to a limited use label license.