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Thermo Scientific™ ActivX™ Azido-FP Serine Hydrolase Probe, 3.5 μg
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Specifications:
| Application | Protein Assays & Analysis, Mass Spectrometry, Chemiluminescent Western Blot Imaging | ||
| Storage Temperature | –80°C | ||
| Product Type | Proteins & Peptides | Forms | Lyophilized |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ ActivX™ Azido-FP Serine Hydrolase Probe is an activity-based fluorophosphonate probe designed for selective labeling of enzymatically active serine hydrolases. The probe contains a reactive fluorophosphonate (FP) group that covalently labels the active-site serine residue of functional serine hydrolases, together with an azido group for downstream detection or enrichment using phosphine- or alkyne-derivatized tags.
This probe is useful for profiling serine hydrolase activity in cells, lysates, tissues, subcellular fractions, and recombinant protein preparations. It supports workflows such as activity-based protein profiling, pull-down enrichment, Western blotting, chemiluminescent detection, fluorescent tagging, and LC-MS/MS-based proteomics.
Key Features
| Feature | Details |
|---|---|
| Activity-Based Probe | Selectively labels enzymatically active serine hydrolases |
| Fluorophosphonate Reactive Group | Covalently targets the active-site serine residue |
| Azido Functional Group | Enables downstream click chemistry or Staudinger-based labeling |
| Multiplex Labeling Capability | Can be used with phosphine- or alkyne-derivatized tags |
| Functional Enzyme Profiling | Detects active enzymes that are biologically relevant at the time of labeling |
| Inhibitor Screening Support | Useful for assessing small molecule binding, active-site inhibition, specificity, and off-target effects |
| Multiple Detection Workflows | Compatible with Western blot, chemiluminescence, mass spectrometry, and fluorescent detection workflows |
Applications
ActivX™ Azido-FP Serine Hydrolase Probe is suitable for:
- Serine hydrolase activity profiling
- Detection of active serine hydrolases in cells and lysates
- Mapping active-site serine residues
- Profiling proteases, lipases, esterases, amidases, and peptidases
- Screening small molecule binding affinities
- Active-site inhibition studies
- Inhibitor target and off-target profiling
- Pull-down enrichment workflows
- Western blot-based enzyme activity studies
- LC-MS/MS-based activity profiling
- Click chemistry-based detection or enrichment workflows
Product Specifications
| Specification | Details |
|---|---|
| Product Name | ActivX™ Azido-FP Serine Hydrolase Probe |
| Catalog Number | 88316 |
| Product Line | ActivX™ |
| Type | Fluorophosphonate, FP probe |
| Target | Serine hydrolase |
| Substrate Type | Serine hydrolase substrate |
| Formulation | Azido-fluorophosphonate |
| Molecular Weight | 350 |
| Label / Dye | Click chemistry label |
| Quantity | 3.5 µg |
| Assay | Protein quantitation assay |
| Application | Pull-down |
| Format | Tube, spin column |
| Detection Method | Chemiluminescence, mass spectrometry, Western blot |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Workflow Overview
| Workflow | Description |
|---|---|
| Active-Site Labeling | FP group covalently labels active-site serine residues in active serine hydrolases |
| Click Chemistry Labeling | Azido group enables reaction with alkyne-derivatized tags for detection or enrichment |
| Staudinger Labeling | Can be used with phosphine-derivatized tags for downstream analysis |
| Western Blot Workflow | Labeled proteins can be detected after tag conjugation and enrichment |
| Mass Spectrometry Workflow | Supports identification and profiling of active serine hydrolases and inhibitor targets |
| Inhibitor Profiling | Competitive labeling can be used to assess inhibitor binding and off-target effects |
Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| 88316 | ActivX™ Azido-FP Serine Hydrolase Probe | 3.5 µg |
Storage & Handling
Store the probe at -80 °C, protected from light. Stock solutions prepared in DMSO can be stored at -20 °C for up to 6 months. Handle using appropriate laboratory personal protective equipment and avoid repeated freeze-thaw cycles where possible.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures. This product is subject to a limited use label license.