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Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH, 5 mL
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Specifications:
| Application | molecular biology, Protein Assays & Analysis | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | C₉H₁₆ClO₆P |
Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH is a ready-to-use, stable 0.5 M solution of tris(2-carboxyethyl)phosphine, commonly known as TCEP. It is a thiol-free, phosphine-based reducing agent designed for efficient reduction of protein and peptide disulfide bonds. Supplied at neutral pH, it is particularly useful as a 10X stock solution for addition to SDS-PAGE sample loading buffers and other protein analysis workflows.
Unlike traditional reducing agents such as DTT and β-mercaptoethanol, Bond-Breaker™ TCEP Solution is odorless, highly stable, resistant to air oxidation, and compatible with many downstream protein applications. Its neutral pH helps maintain sharp electrophoretic bands while avoiding the acidic conditions associated with TCEP-HCl.
Key Features
| Feature | Description |
|---|---|
| Thiol-free reducing agent | Does not contain sulfhydryl groups, reducing interference in many downstream applications |
| Odorless formulation | Safer and more pleasant to handle compared with β-mercaptoethanol |
| Stable solution | Resistant to air oxidation and suitable for room-temperature storage |
| Fast reduction | Reduces protein disulfide bonds within minutes under appropriate conditions |
| Broad compatibility | Effective across a wide pH, temperature, detergent, and salt range |
| Neutral pH | Helps prevent protein hydrolysis and carbohydrate modification associated with acidic TCEP-HCl |
| Convenient 0.5 M concentration | Suitable as a 10X stock for SDS-PAGE sample buffers |
Applications
Bond-Breaker™ TCEP Solution is suitable for:
| Application Area | Use |
|---|---|
| SDS-PAGE sample preparation | Reduction of disulfide bonds before electrophoresis |
| Protein analysis | Preparation of reduced protein samples |
| Peptide and protein chemistry | Reduction of cystine/disulfide-containing peptides and proteins |
| His-tagged protein purification workflows | Compatible with many downstream purification procedures |
| Maleimide conjugation workflows | Useful where thiol-free reduction is preferred |
| General molecular biology research | Replacement for DTT or β-mercaptoethanol in many reducing applications |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH |
| Catalog Number | 77720 |
| Product Type | Disulfide Reducing Reagent |
| Product Line | Bond-Breaker™ |
| Form | Liquid |
| Concentration | 0.5 M |
| Quantity | 5 mL |
| Recommended Working Range | 5–50 mM TCEP |
| Effective pH Range | pH 4.0–9.0 |
| Temperature Range for Reduction | 5–95°C |
| Storage | Store at room temperature |
| Unit Size | Each |
| Use Statement | For Research Use Only. Not for use in diagnostic procedures |
Usage Considerations
Reduction can occur efficiently over a broad pH and temperature range. Most proteins can be reduced without a denaturant, although a denaturant such as guanidine hydrochloride may help expose internal disulfide bonds. Urea is not recommended because it can form cyanates that react with sulfhydryl groups.
Metal contact should be avoided because metals can reduce TCEP activity. Including 5–20 mM EDTA in the sample buffer can help prevent reoxidation of reduced sulfhydryl groups by divalent metals such as zinc, copper, and magnesium. Reduced samples should ideally be used immediately because disulfide bonds may reform over time.
Related Products
| Product | Description |
|---|---|
| Pierce™ TCEP-HCl | Solid TCEP hydrochloride reducing agent |
| Pierce™ DTT | Traditional thiol-based reducing agent for protein sample preparation |
Summary
Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH provides a convenient, stable, odorless, and thiol-free alternative to DTT and β-mercaptoethanol for reducing protein and peptide disulfide bonds. Its neutral pH formulation, broad compatibility, and ready-to-use 0.5 M concentration make it well suited for SDS-PAGE sample preparation, protein analysis, purification workflows, and other research applications requiring reliable disulfide bond reduction.