0
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ DMEM/F-12, No Phenol Red is a high-quality basal medium formulated to support the growth and maintenance of a wide range of mammalian cell types. This phenol red-free formulation is specifically designed for applications where color indicators may interfere with experimental results, such as fluorescence imaging, hormone-response studies, and spectrophotometric assays.
DMEM/F-12 combines the nutrient-rich profile of Dulbecco’s Modified Eagle Medium (DMEM) with the broader component spectrum of Ham’s F-12 in a 1:1 ratio, delivering a balanced environment for robust cell growth and metabolic activity.
Key Features
- Phenol Red-Free – Ideal for fluorescence and color-sensitive assays
- Contains L-Glutamine – Supports optimal cell metabolism
- High Glucose Formulation – Promotes strong cell proliferation
- Includes Sodium Pyruvate – Additional energy source
- Animal Origin-Free
- Sterile-Filtered for Reliability
- cGMP-Compliant (ISO 13485)
- Research Use Only (RUO)
Formulation Overview
Included Additives
✔ L-Glutamine
✔ High Glucose
✔ Sodium Pyruvate
Excluded Components
✘ No Phenol Red
✘ No HEPES
✘ No proteins, lipids, or growth factors
Supplementation Required:
Typically supplemented with 10% Fetal Bovine Serum (FBS) depending on the cell line.
Culture Conditions
- Buffer System: Sodium bicarbonate
- CO₂ Requirement: 5–10% CO₂ incubator
- Storage: 2–8°C, protect from light
- Shipping: Ambient
- Shelf Life: 12 months from manufacture
Supported Cell Lines
Successfully used for culturing:
- MDCK cells
- Glial cells
- Fibroblasts
- Human endothelial cells
- Rat fibroblasts
Applications
- Fluorescence microscopy and imaging
- Hormone-sensitive cell assays
- Reporter gene assays
- Spectrophotometric measurements
- General mammalian cell culture research
Gibco™ DMEM/F-12, No Phenol Red provides researchers with a versatile and dependable basal medium while eliminating the potential interference associated with phenol red. By combining robust nutritional support with compatibility for sensitive analytical applications, this formulation offers enhanced experimental flexibility without compromising cell growth performance.