Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Column

The Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Column is a high-performance nano-LC column engineered for advanced peptide mapping and proteomics workflows. Designed to deliver exceptional separation efficiency, reproducibility, and seamless LC-MS integration, these columns are optimized for use in bottom-up proteomics and high-sensitivity LC-MS applications.

Built with ultrapure, fully porous silica particles (2 μm, C18) and packed under ultra-high-pressure conditions, PepMap Neo columns offer superior peak symmetry, enhanced resolution, and increased sample capacity. With a maximum pressure tolerance of 1500 bar (22,000 psi), they are ideally suited for modern UHPLC systems, enabling the use of longer columns and higher efficiency separations.

The innovative EASY-Spray™ Click-and-Spray format ensures perfect, reproducible connections to compatible mass spectrometers, eliminating dead volume and reducing variability. This results in highly stable nano-electrospray performance, improved sensitivity, and consistent results across experiments and laboratories.

Available Configurations

Key Features

• Integrated EASY-Spray™ column-emitter design for seamless LC-MS connectivity
• Click-and-Spray format ensures reliable, zero-dead-volume connections
• Ultra-high-pressure capability (up to 1500 bar / 22,000 psi)
• C18 reversed-phase chemistry optimized for peptide separations
• 2 μm fully porous silica particles for high efficiency and resolution
• Exceptional column-to-column reproducibility for consistent results
• Enhanced peak symmetry and chromatographic performance
• High sample capacity and improved proteome coverage
• Compatible with nano-, capillary-, and micro-flow LC-MS workflows
• Designed for high-sensitivity proteomics applications

Applications

The EASY-Spray PepMap Neo UHPLC Column is ideal for:

• Bottom-up proteomics workflows
• Peptide mapping and protein identification
• Post-translational modification (PTM) analysis
• Biopharmaceutical characterization
• Nano LC-MS analysis of complex samples
• High-resolution peptide separations
• Quantitative and qualitative proteomics studies

Technical Specifications

Unique Advantages

The PepMap Neo UHPLC Column combines state-of-the-art packing technology with a fully integrated nano-ESI interface, ensuring unmatched chromatographic performance and reproducibility. Its high-pressure capability allows researchers to achieve greater separation efficiency and deeper proteome coverage, especially when working with complex biological samples.

The Click-and-Spray EASY-Spray design eliminates common issues such as poor connections and dead volume, ensuring stable electrospray ionization and consistent analytical results. This makes the column particularly valuable for high-throughput proteomics studies, multi-site collaborations, and applications requiring high confidence in data reproducibility.

The Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Column is a premium solution for laboratories seeking high-resolution, reproducible peptide separations in LC-MS workflows. With its advanced column design, ultra-high-pressure capability, and seamless MS integration, it enables researchers to achieve greater analytical depth, sensitivity, and confidence in proteomics and biopharma applications.