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Virplex® OROPOUCHE–MAYARO Virus Real-Time PCR Kit, 3-Plex, 24 Rxn
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Target Pathogens: Oropouche virus (OROV), Mayaro virus (MAYV)
Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® OROPOUCHE–MAYARO Virus Real-Time PCR Kit is an advanced RT-qPCR assay designed for the qualitative detection of nucleic acids from Oropouche virus (OROV) and Mayaro virus (MAYV) in human serum and plasma samples.
These emerging arboviruses, transmitted primarily by mosquitoes and midges, are responsible for febrile illnesses similar to dengue and chikungunya, making rapid molecular detection crucial for differential diagnosis and outbreak surveillance.
This kit enables simultaneous multiplex detection of both viruses in a single reaction tube, offering fast, accurate, and reliable results for clinical and research laboratories.
Key Features
- Dual-Pathogen Detection – Simultaneous identification of Oropouche and Mayaro viruses.
- Multiplex RT-PCR Design – 3-target detection in a single reaction tube per sample.
- Internal Control (MS2 RNA Phage) – Ensures amplification integrity and detects inhibitors or setup errors.
- Flexible Format – 24 reactions divided into 3 strips of 8 wells for easy batch management.
- Fast Turnaround – Delivers results in under 2 hours.
- Stable & Convenient – Lyophilized master mix and positive control reduce cold-chain requirements.
- Instrument Compatibility – Suitable for 3-channel real-time PCR cyclers.
- Research Use Only (RUO) – For research, surveillance, and epidemiological applications.
Kit Contents (24 Reactions)
- Lyophilized Master Mix (Multiplex format)
- Internal Control (MS2 RNA)
- Positive Control
- Pre-dispensed Strips (3 × 8 wells)
- User Manual
Technical Specifications
| Parameter | Details |
|---|---|
| Product Name | Virplex® OROPOUCHE–MAYARO Virus Real-Time PCR Kit |
| Catalogue Number | RTPCR032-LP-R |
| Format | 24 Tests |
| Target Pathogens | Oropouche virus (OROV), Mayaro virus (MAYV) |
| Class | RUO (Research Use Only) |
| Reaction Setup | One single reaction tube per sample |
| Internal Control | MS2 RNA bacteriophage |
| Sample Types | Human serum and plasma |
| Cycler Compatibility | ≥ 3-channel real-time PCR instruments |
| Reagent Form | Lyophilized |
| Time to Results | < 2 hours |
Related Products
- Virplex® ZIKV/DENV/CHIKV Real-Time PCR Kit
- AMPLIRUN® TOTAL ZIKV/DENV/CHIKV Control
- AMPLIRUN® DENGUE RNA Control
Applications
- Arbovirus Detection & Surveillance – Differentiating Oropouche and Mayaro from other febrile viral infections.
- Public Health Research – Monitoring emerging tropical arboviruses in endemic regions.
- Clinical Virology Studies – Characterization of dual or cross infections.
- Epidemiological Investigations – Tracking outbreaks and vector-borne virus spread.
Ordering Information
| Product | Catalogue Number | Pack Size | Classification |
|---|---|---|---|
| Virplex® OROPOUCHE–MAYARO Virus Real-Time PCR Kit | RTPCR032-LP-R | 24 Tests | RUO |
The Virplex® OROPOUCHE–MAYARO Virus Real-Time PCR Kit (RTPCR032-LP-R) provides a sensitive, rapid, and reliable molecular tool for detecting two emerging arboviruses with significant public health relevance. With its MS2 RNA internal control, lyophilized reagents, and 3-target multiplex format, it is an ideal solution for laboratories conducting arboviral research, differential diagnosis, and epidemiological surveillance in tropical and endemic areas.