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Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® ZIKV/DENV/CHIKV RealTime PCR Kit is a multiplex real-time RT-PCR assay designed for the simultaneous detection of Zika virus (ZIKV), Dengue virus (DENV), and Chikungunya virus (CHIKV) in human serum and plasma samples. This assay performs reverse transcription and amplification of nucleic acids in a single step, providing fast, reliable, and cost-efficient results.
Available Kits
| Product | Reference | Nº Tests | Class |
|---|---|---|---|
| ZIKV/DENV/CHIKV RealTime PCR Kit | RTPCR004-LP | 96 | CE0123 IVDR |
| ZIKV/DENV/CHIKV RealTime PCR Kit | RTPCR004 | 48 | CE0123 IVDR |
Key Features
- Multiplex detection of 4 targets – detects Zika, Dengue, and Chikungunya viruses in a single reaction tube per sample.
- Internal control (MS2 RNA bacteriophage) – monitors sample RNA integrity, inhibition carry-over, and proper assay setup.
- Compatible with 4-channel qPCR cyclers – FAM, HEX (VIC), Texas Red (ROX), and Cy5.
- Fast and reliable turnaround – results in under 2 hours.
- Stability & convenience – lyophilized master mix and positive control included, minimizing cold chain requirements.
- Flexible formats – available in vial-based and pre-dispensed strip presentations to suit different laboratory workflows.
Applications
- Simultaneous detection of ZIKV, DENV, and CHIKV in human serum or plasma.
- Differential diagnosis of arboviral infections with overlapping clinical symptoms.
- Validation of in-house and commercial multiplex arbovirus diagnostic workflows.
- Public health outbreak monitoring and vector-borne disease surveillance.
- Quality control for qPCR laboratories detecting mosquito-borne viral infections.
Product Details
| Property | Value |
|---|---|
| Sample Types | Human serum, plasma |
| Targets | Zika virus (ZIKV), Dengue virus (DENV), Chikungunya virus (CHIKV) |
| Detection Format | Multiplex RT-qPCR (4 channels: FAM, HEX/VIC, Texas Red/ROX, Cy5) |
| Nº Tests | 48 or 96 (kit dependent) |
| Internal Control | MS2 RNA bacteriophage |
| Result Time | < 2 hours |
| Reagents | Lyophilized master mix + positive control |
| Class | CE0123 IVDR |
The Virplex® ZIKV/DENV/CHIKV RealTime PCR Kit offers a rapid, accurate, and efficient solution for the multiplex detection of arboviruses. By combining Zika, Dengue, and Chikungunya virus targets into a single assay with integrated controls, this kit is ideal for clinical diagnostics, outbreak surveillance, and molecular virology research in endemic regions.
- Reactions: 96 Rxn 48 rxn