Invitrogen™ Rnalater™ Stabilization Solution, 500Ml

Invitrogen™ RNAlater™ Stabilization Solution is a ready-to-use aqueous tissue storage reagent designed to stabilize and protect cellular RNA in intact, unfrozen tissue samples. It eliminates the need for immediate RNA extraction or snap-freezing in liquid nitrogen, making it ideal for field collection, clinical sampling, and research workflows.

For Research Use Only. Not for use in diagnostic procedures.

Key Advantages

• Effective RNA Stabilization
  • 1 day at 37°C
  • 1 week at 25°C
  • 1 month at 4°C
  • Indefinite storage at -20°C
• Immediate RNase Inactivation
  Rapidly permeates tissues and inactivates RNases to preserve RNA integrity.
• No Liquid Nitrogen Required
  Eliminates the need for snap-freezing or urgent processing.
• Field-Friendly
  Ideal for hospital collection sites, remote sampling locations, and research expeditions.
• Broad Compatibility
  Works with most RNA isolation methods and downstream applications.

Applications

RNAlater™ has been tested with:

• Mammalian tissues
• Plant tissues
• E. coli
• Yeast
• Fish
• Xenopus
• Drosophila
• Cultured cells

It is particularly useful for:

• Preserving RNA in RNase-rich tissues
• Time-course sampling experiments
• Archiving tissues for later microdissection
• Stabilizing RNA during long dissections
• Sample shipment on wet ice or overnight at room temperature

Procedure Overview

1. Dissect tissue into pieces less than 0.5 cm in any dimension.
2. Submerge in approximately 5 volumes of RNAlater at room temperature.
3. Store according to required conditions.
4. Remove tissue and proceed directly to RNA isolation.

Tissues treated with RNAlater can be:

• Homogenized directly in lysis buffer
• Frozen and ground with mortar and pestle
• Thawed and processed like fresh tissue

RNases remain inactivated during processing.

Compatibility with RNA Isolation Methods

RNAlater™ is compatible with:

• TRIzol™ reagent
• Silica column methods (e.g., RNeasy-style kits)
• Acid phenol extraction
• Oligo(dT) mRNA purification workflows

Use of RNAlater does not negatively impact downstream gene expression analysis compared to conventional processing methods.

Product Specifications

Invitrogen RNAlater™ Stabilization Solution provides a reliable, field-friendly method for preserving RNA integrity in biological samples without freezing. By immediately inactivating RNases and stabilizing RNA within intact tissues, it supports high-quality RNA isolation and downstream molecular biology applications including RT-qPCR, RNA sequencing, and gene expression analysis.