Thermo Scientific™ EASY-ETD Option for Orbitrap™ Excedion™ Pro Mass Spectrometer

The Thermo Scientific™ EASY-ETD Option is an advanced fragmentation upgrade designed for the Orbitrap™ Excedion™ Pro Mass Spectrometer, enabling fast, sensitive, and highly informative Electron Transfer Dissociation (ETD) and Electron-Transfer/Higher-Energy Collision Dissociation (EThcD) workflows. Available as a factory-installed option or field upgrade, EASY-ETD significantly expands the analytical capabilities of the Orbitrap Excedion Pro, particularly for proteomics, structural biology, and biopharmaceutical characterization.

This module introduces radical-driven fragmentation chemistry, allowing efficient interaction of precursor ions with fluoranthene radical anions (m/z 202) within the ion routing multipole (IRM). The result is the generation of c- and z-type fragment ions, which are essential for preserving labile post-translational modifications (PTMs) and enabling detailed peptide and protein sequencing.

By combining ETD with Higher-Energy Collision Dissociation (HCD) in EThcD mode, the system produces complementary fragmentation patterns, improving sequence coverage, confidence in identification, and structural resolution of complex biomolecules.

Key Features

• Advanced ETD fragmentation capability for Orbitrap Excedion Pro systems
• Available as factory installation or field upgrade
• Enables fast and highly sensitive electron transfer reactions
• Utilizes fluoranthene radical anions (m/z 202) for efficient ion fragmentation
• Generates c/z ion series for detailed peptide backbone analysis
• Supports EThcD fragmentation, combining ETD and HCD for enhanced structural insights
• Operates within the ion routing multipole (IRM) for controlled fragmentation
• Preserves labile PTMs such as phosphorylation and glycosylation
• Enhances top-down, middle-down, and bottom-up proteomics workflows
• Improves sequence coverage and confidence in protein identification

Applications

The EASY-ETD option is ideal for advanced analytical workflows, including:

• Proteomics and deep protein sequencing
• Post-translational modification (PTM) analysis (phosphorylation, glycosylation, acetylation)
• Top-down and middle-down proteomics
• Immunopeptidomics and peptide mapping
• Biopharmaceutical characterization
• Structural biology and conformational studies
• Hydrogen-Deuterium Exchange (HDX-MS) workflows
• Analysis of labile biomolecules requiring gentle fragmentation

Technical Specifications

Unique Advantages

The EASY-ETD option provides a critical enhancement for laboratories requiring high-confidence structural characterization of proteins and peptides. Unlike traditional collision-based fragmentation methods, ETD preserves fragile modifications, making it indispensable for PTM analysis and intact protein studies.

Its integration with EThcD workflows delivers complementary fragmentation data, enabling comprehensive sequence coverage and improved identification of complex biomolecules. This makes the EASY-ETD module particularly valuable in biopharma characterization, advanced proteomics, and structural biology research, where accuracy and depth of analysis are essential.

The Thermo Scientific™ EASY-ETD Option for Orbitrap™ Excedion™ Pro is a powerful upgrade that unlocks advanced fragmentation capabilities, allowing researchers to perform more detailed, accurate, and confident molecular characterization. Whether applied in proteomics, PTM analysis, or structural biology, this module enhances the performance of the Orbitrap Excedion Pro, making it a future-ready platform for cutting-edge mass spectrometry applications.