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Human Immunoglobulin A (IgA) ELISA Kit
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Wavelength: 450nm
Assay range: 4ng/ml-200ng/ml
Sensitivity: 0.8ng/ml
Standard: 270ng/ml
Sample Volume: 10ul
Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Grade | Molecular Biology | ||
The Human Immunoglobulin A (IgA) ELISA Kit is a high-performance sandwich ELISA designed for the quantitative detection of IgA in human samples. It is validated for use with serum, plasma, cell culture supernatants, body fluids, and tissue homogenates, providing broad flexibility for immunology and biomedical research.
IgA plays a critical role in mucosal immunity, serving as the first line of defense against pathogens in the respiratory and gastrointestinal tracts. Abnormal IgA levels are associated with autoimmune disorders, kidney disease, chronic infections, and immune deficiency syndromes, making this kit an essential research tool.
Key Features
- High Sensitivity – Detects as low as 0.8 ng/mL.
- Broad Assay Range – 4 – 200 ng/mL.
- Reliable Precision – Intra-assay CV < 10%, Inter-assay CV < 12%.
- Flexible Pack Sizes – Available in 48T and 96T formats.
- Comprehensive Kit – Pre-coated plate, standards, conjugates, diluents, buffers, substrate, and stop solution included.
- Research Grade – For research use only (RUO); not for diagnostic purposes.
Kit Contents (Universal)
- Pre-coated MicroELISA Strip Plate (48 or 96 wells depending on kit size)
- Standards (270 ng/mL) & Standard Diluent
- HRP-Conjugate Reagent
- Sample Diluent
- Chromogen Solutions A & B
- Stop Solution
- Wash Buffer (Concentrate)
- Closure Plate Membrane
- Sealed Storage Bag
- User Manual
(Reagent volumes are scaled according to 48T or 96T format.)
Technical Specifications
| Parameter | Details |
|---|---|
| Catalogue Numbers | SL0912Hu-48 (48T), SL0912Hu (96T) |
| Assay Type | Sandwich ELISA |
| Detection Range | 4 – 200 ng/mL |
| Sensitivity | 0.8 ng/mL |
| Precision | Intra-assay CV < 10%, Inter-assay CV < 12% |
| Sample Types | Serum, plasma, body fluids, culture supernatants, tissue homogenates |
| Wavelength | 450 nm |
| Storage | 2–8 °C |
| Shelf Life | 6 months |
| Use | Research Use Only (RUO) |
Applications
- Mucosal Immunity Research – Quantifying IgA as a frontline immune response biomarker.
- Autoimmune Disorders – Monitoring IgA in systemic and mucosal autoimmune diseases.
- Renal Disease Research – Investigating IgA nephropathy and immune complex disorders.
- Infectious Disease Studies – Tracking IgA response to bacterial, viral, and parasitic infections.
- Immunodeficiency Research – Evaluating IgA deficiency in immune-compromised subjects.
Storage & Handling
- Store reagents at 2–8 °C.
- Shelf life: 6 months.
- Avoid repeated freeze–thaw cycles.
- Do not use with sodium azide (NaN₃), as it inhibits HRP activity.
Ordering Information
| Product | Catalogue Number | Pack Size | Storage | Validity |
|---|---|---|---|---|
| Human Immunoglobulin A (IgA) ELISA Kit | SL0912Hu-48 | 48 Tests | 2–8 °C | 6 months |
| Human Immunoglobulin A (IgA) ELISA Kit | SL0912Hu | 96 Tests | 2–8 °C | 6 months |
The Human Immunoglobulin A (IgA) ELISA Kit (Cat. No. SL0912Hu-48 & SL0912Hu) provides researchers with a sensitive, accurate, and reproducible method for measuring IgA levels in human samples. With broad sample compatibility, robust assay performance, and availability in both 48-test and 96-test formats, this kit is a valuable tool in studies of mucosal immunity, autoimmune disease, renal pathology, and infectious disease research.
- Pack Size: For 48 Tests For 96 Tests