Human Immunoglobulin Free Light Chains Lambda (FLC-IgL) ELISA Kit

The Human Immunoglobulin Free Light Chains Lambda (FLC-IgL) ELISA Kit is a sensitive, high-quality sandwich ELISA designed for the quantitative detection of free lambda light chains (FLC-IgL) in human biological fluids. It is validated for use with serum, plasma, urine, cerebrospinal fluid, pleural/peritoneal fluid, culture supernatants, and tissue homogenates.

Abnormal levels of free lambda chains, along with kappa chains, are widely studied as biomarkers in multiple myeloma, B-cell malignancies, kidney dysfunction, autoimmune diseases, and immunodeficiency syndromes. This kit provides researchers with an accurate and reproducible solution for immunoglobulin light chain quantification.

Key Features

• High Sensitivity – Detects as low as 8.9 pg/mL.
• Broad Assay Range – 44.8 pg/mL – 4000 pg/mL.
• Reliable Precision – Intra-assay CV < 10%, Inter-assay CV < 12%.
• Two Pack Sizes – Available in 48T and 96T formats for different project needs.
• Wide Sample Compatibility – Works with serum, plasma, CSF, urine, tissue extracts, and cell culture fluids.
• Comprehensive Kit – Pre-coated plate, standards, HRP conjugate, diluents, buffers, substrates, and stop solution included.
• Research Use Only – Not intended for diagnostic or therapeutic purposes.

Kit Contents (Universal)

• Pre-coated MicroELISA Strip Plate (48 or 96 wells depending on kit size)
• Standards (5400 pg/mL) & Standard Diluent
• HRP-Conjugate Reagent
• Sample Diluent
• Chromogen Solutions A & B
• Stop Solution
• Wash Buffer (Concentrate)
• Closure Plate Membrane
• Sealed Storage Bag
• User Manual

(Reagent volumes are scaled according to 48T or 96T format.)

Technical Specifications

Applications

• Multiple Myeloma Research – Measurement of free lambda chains as critical disease biomarkers.
• B-cell Malignancies – Understanding immunoglobulin dysregulation in hematological cancers.
• Immunology Studies – Investigating autoimmune and immunodeficiency disorders.
• Renal and Inflammatory Research – Studying the role of free light chains in kidney and systemic disease models.

Storage & Handling

• Store reagents at 2–8 °C.
• Shelf life: 6 months.
• Avoid repeated freeze–thaw cycles.
• Do not use with sodium azide (NaN₃), as it inhibits HRP activity.

Ordering Information

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The Human Immunoglobulin Free Light Chains Lambda (FLC-IgL) ELISA Kit (Cat. No. SL4547Hu-48 & SL4547Hu) provides a robust, sensitive, and reproducible platform for quantifying free lambda light chains in human samples. With its broad sample flexibility, high assay precision, and availability in both 48-test and 96-test formats, this kit is a reliable choice for researchers in oncology, immunology, nephrology, and autoimmune disease research. It empowers laboratories with consistent performance and reliable results in critical free light chain studies.