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Proteintech™ ETV4 Polyclonal Antibody, 150 µL
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Specifications:
| Application | Western Blot, immunocytochemistry, Immunohistochemistry | ||
| Storage Temperature | -20°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Proteintech | ||
| Product Grade | Molecular Biology | ||
The Proteintech ETV4 Polyclonal Antibody is a high-quality antibody developed for the detection of ETV4 (ETS Variant Transcription Factor 4), also known as PEA3 (E1A enhancer-binding protein). ETV4 is a member of the ETS family of transcription factors and plays a critical role in gene regulation, cell proliferation, differentiation, and tumor progression.
This antibody is raised against a recombinant fusion protein corresponding to amino acids 1–207 of ETV4, providing strong specificity for detecting endogenous ETV4 in human, mouse, and rat samples. It has been validated for Western blotting, immunocytochemistry (ICC/IF), and immunohistochemistry (IHC), making it suitable for a wide range of molecular and cellular biology applications.
Key Features
- Specific for ETV4 / PEA3 transcription factor
- Polyclonal antibody for enhanced epitope recognition
- Reactivity: Human, Mouse, Rat
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Validated for:
- Western Blot (WB): 1:500–1:1,000
- Immunocytochemistry (ICC/IF)
- Immunohistochemistry (IHC)
- Targets ETS family DNA-binding protein
- Supplied as unconjugated liquid antibody
- Purified by antigen affinity chromatography
- Stable formulation in PBS with 50% glycerol
Applications
This antibody is ideal for:
- Western blot analysis of ETV4 expression
- Immunofluorescence imaging in cultured cells (ICC/IF)
- Immunohistochemical detection in tissue samples
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Studies of:
- Transcriptional regulation mechanisms
- ETS family transcription factors
- Cancer progression and metastasis
- Gene expression and signaling pathways
- Epithelial and fibroblast cell biology
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Proteintech ETV4 Polyclonal Antibody |
| Catalog Number | 10684-1-AP |
| Type | Antibody |
| Class | Polyclonal |
| Host / Isotype | Rabbit / IgG |
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Amount | 37.5 µg |
| Volume | 150 µL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Preservative | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Immunogen Information
| Parameter | Details |
|---|---|
| Immunogen | ETV4 Fusion Protein Ag0984 |
| Immunogen Region | 1–207 aa |
| Encoded By | BC007242 |
Target Information
ETV4 (ETS Variant 4) is a transcription factor belonging to the ETS gene family, which is characterized by its ability to bind DNA sequences containing a central GGAA motif. ETV4 regulates the transcription of genes involved in cell proliferation, differentiation, and invasion.
ETV4 is primarily expressed in epithelial and fibroblastic cells and has been implicated in tumor progression and metastasis in several cancers. Unlike other ETS family members, ETV4 shows limited expression in hematopoietic cells, making it particularly relevant for studying epithelial-derived tumors and signaling pathways.
Advantages
- High sensitivity due to polyclonal epitope recognition
- Suitable for multiple applications (WB, IHC, ICC)
- Broad species reactivity for versatile research use
- Ideal for transcription factor and cancer research
- Reliable performance in protein expression studies
The Proteintech ETV4 Polyclonal Antibody is a versatile and reliable reagent for investigating ETS transcription factor biology, gene regulation, and cancer-related pathways. Its validated performance across multiple applications and strong specificity make it an excellent choice for molecular biology and oncology research laboratories.