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Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads
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Specifications:
| Application | molecular biology, Magnetic Affinity Separation | ||
| Storage Temperature | 2-8°C | ||
| Product Type | ImmunoMagnetic Separation Products | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast and convenient magnetic affinity purification method for His-tagged recombinant proteins. The beads are designed for both manual benchtop purification and automated high-throughput workflows using magnetic particle processors such as the Thermo Scientific™ KingFisher™ Flex system.
The beads are incubated with clarified cell lysate containing the His-tagged target protein, magnetically separated from the supernatant, washed to remove nonspecifically bound proteins, and then eluted using an appropriate elution buffer. This workflow supports rapid purification, screening of expression conditions, and small-scale recombinant protein purification.
Key Features
| Feature | Benefit |
|---|---|
| High binding capacity | Binds more than 70 mg of 6xHis-tagged protein per mL of settled resin |
| Magnetic agarose format | Enables fast separation without centrifugation or column packing |
| Ni-NTA affinity chemistry | Supports selective purification of polyhistidine-tagged proteins |
| Low nonspecific binding | Optimized protocol helps improve purity of recovered tagged proteins |
| Manual or automated use | Suitable for benchtop magnetic stands and KingFisher™ magnetic processors |
| Native or denaturing purification | Provides flexibility for different recombinant protein workflows |
| Compatible with common lysis reagents | Can be used with Thermo Scientific™ cell lysis reagents and compatible buffer additives |
| Superparamagnetic bead design | Supports uniform handling and reproducible purification performance |
Ordering Information
| Catalog Number | Supplied Quantity | Settled Resin Volume | Suspension Format |
|---|---|---|---|
| 78605 | 4 mL | 1 mL settled resin | 25% v/v suspension in 20% ethanol |
| 78606 | 20 mL | 5 mL settled resin | 25% v/v suspension in 20% ethanol |
Applications
Pierce™ Ni-NTA Magnetic Agarose Beads are suitable for:
- His-tagged recombinant protein purification
- Small-scale affinity purification
- Expression screening
- Purification condition optimization
- High-throughput protein purification workflows
- Recombinant protein research
- Native and denaturing protein purification
- Manual benchtop purification using magnetic stands
- Automated purification using KingFisher™ platforms
Technology Overview
The beads consist of highly crosslinked agarose embedded with magnetite particles and functionalized with a covalently attached nitrilotriacetic acid, NTA, chelator charged with divalent nickel ions. The nickel-charged NTA ligand interacts with histidine residues in His-tagged proteins, allowing selective capture and purification from cell lysates.
This format combines the binding performance of traditional Ni-NTA agarose resins with the convenience of magnetic separation, making it useful for rapid workflows and higher-throughput screening.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads |
| Product Line | Pierce™ |
| Type | Magnetic Agarose Bead |
| Format | Bottle |
| Form | Liquid suspension |
| Slurry Concentration | 25% |
| Bed Volume | 1 mL, depending on catalog option |
| Particle Size | 30 µm |
| Stationary Phase | Metal chelate, divalent nickel |
| Protein Form | Recombinant |
| Binding Capacity | More than 70 mg 6xHis-tagged protein per mL settled resin |
| Shipping Condition | Wet ice |
| Storage | 2–8°C |
| Unit Size | Each |
Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads are high-capacity magnetic affinity beads for fast and reproducible purification of His-tagged recombinant proteins. Their Ni-NTA chemistry, low nonspecific binding, magnetic handling, and compatibility with manual or automated workflows make them ideal for small-scale protein purification, screening studies, and high-throughput recombinant protein workflows.
- Pack Size: 20 mL 4 mL