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Thermo Scientific™ DyLight™ Microscale Antibody Labeling Kit
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Specifications:
| Application | Western Blot, ELISA-Based Assays, Immunofluorescence, Fluorescence-Based Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ DyLight™ Microscale Antibody Labeling Kits provide a complete, optimized solution for fluorescent labeling of small quantities of antibody or protein (100 μg per reaction) with DyLight™ NHS ester fluorophores. These microscale kits are ideal for research workflows that require multiple small-scale labeling experiments for applications such as immunofluorescence microscopy, flow cytometry, Western blotting, ELISA, and IHC.
Each kit contains pre-measured, single-use vials of amine-reactive DyLight dye, as well as all necessary buffers, purification resin, and spin columns. Labeled antibodies are typically ready in 90 minutes with minimal hands-on time and are suitable for direct use in fluorescence-based detection assays.
Key Features and Benefits
Optimized for Small-Scale Labeling
- Designed to label 100 μg of IgG or protein per reaction.
- Perform up to 5 labeling reactions per kit with consistent results.
Bright, Stable Fluorescent Signal
- DyLight dyes provide high fluorescence intensity and superior photostability across pH 4–9.
- Comparable to or brighter than CyDye and LI-COR™ dyes in most applications.
Reliable Amine-Reactive Chemistry
- NHS-ester-activated dyes form stable amide bonds with primary amines on lysine residues or protein N-termini.
- Specific labeling with minimal nonspecific modification.
Convenient All-in-One Kit Format
- Includes pre-aliquoted dye vials, borate buffer, purification resin, spin columns, and collection tubes.
- No need to weigh reagents or source additional components.
Applications
- Immunofluorescence microscopy (IF)
- Flow cytometry and cell surface staining
- Immunohistochemistry (IHC)
- Western blot detection
- ELISA fluorescence detection
- In vitro and in vivo protein labeling
Technical Specifications
| Property | Details |
|---|---|
| Catalog Numbers | See table below |
| Labeling Scale | 100 μg per reaction |
| Reactions per Kit | 5 |
| Label Type | DyLight™ Fluor NHS Ester |
| Reactive Moiety | NHS Ester (Amine-reactive) |
| Target Functional Group | Primary amines (–NH₂) |
| Excitation/Emission | Varies by fluorophore (see table) |
| Solubility | Water-compatible workflow |
| Storage Conditions | Dye: –20°C; Other components: 4°C |
| Shipping Condition | Ambient temperature |
| Kit Contents | 5 × 15 μg DyLight NHS Ester, 1 mL Borate Buffer (0.67 M), 5 mL Purification Resin, 5 Spin Columns, 10 Collection Tubes |
| Unit Size | Each kit (5 reactions) |
Available DyLight™ Microscale Kits
| Catalog Number | Excitation / Emission (nm) | Fluorophore |
|---|---|---|
| 53025 | 490 / 525 | DyLight 488 |
| 53021 | 401 / 421 | DyLight 405 |
| 53045 | 590 / 617 | DyLight 594 |
| 53063 | 784 / 814 | DyLight 800 |
| 84531 | 562 / 576 | DyLight 550 |
| 84536 | 652 / 672 | DyLight 650 |
| 84539 | 749 / 775 | DyLight 755 |
DyLight™ Microscale Antibody Labeling Kits offer a flexible and efficient approach for fluorescently labeling small quantities of antibodies or proteins. Ideal for research labs needing high-performance fluorophores in microscale formats, these kits deliver reproducible, bright conjugates ready for immediate use in fluorescence-based applications.
- Fluorophore: DyLight™ 488 DyLight™ 405 DyLight™ 594 DyLight™ 800 DyLight™ 550 DyLight™ 650 DyLight™ 755