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Sigma-Aldrich™ Fast Garnet GBC Sulfate Salt (Diazonium Dye)
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Specifications:
| Application | Analytical Chemistry, Biochemical Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Analytical grade | Formula | C₁₄H₁₄N₄O₄S |
Fast Garnet GBC is a high-performance diazonium dye widely used in azo-dye histochemical methods, particularly for alkaline phosphatase staining, non-specific esterase activity assays, and enzyme localization in tissues and microbial samples. Upon reaction with α-naphthol or naphthol AS substrates, it produces a deep red, insoluble azo dye precisely at the site of enzymatic activity—ensuring excellent localization, sensitivity, and contrast.
This reagent is a trusted choice for professional histology, hematology, cytochemistry, and diagnostic assay manufacturing laboratories requiring reliable, reproducible staining outcomes.
Key Features
- High dye content (≥90%) for consistent and reproducible staining.
- Forms intense red azo dye on coupling with naphthol derivatives.
- Widely used for alkaline phosphatase, AST, and esterase activity staining.
- Water-soluble (5 mg/mL, clear solution).
- Stable under refrigerated conditions (2–8 °C) for long shelf life.
- Suitable for use in diagnostic assay production and research workflows.
- Available in 1 g, 5 g, and 25 g high-purity powder formats.
Applications
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Alkaline Phosphatase Staining
Produces a red insoluble azo dye at sites of enzyme activity when used with α-naphthol. -
Aspartate Aminotransferase (AST) Staining
Provides strong color intensity and high enzyme localization accuracy. -
Non-Specific Esterase Detection
Essential for immunology, cytochemistry, and blood cell typing workflows. -
Diagnostic Assay Manufacturing
Incorporated into kit formulations for enzyme visualization. -
Histology & Hematology
Suitable for tissue sections, cytospins, and cell suspensions.
Technical Specifications
| Property | Value |
|---|---|
| Product Name | Fast Garnet GBC sulfate salt (Diazonium dye) |
| Synonyms | 2-Methyl-4-([2-methylphenyl]azo)benzenediazonium salt; Azoic Diazo No. 4; 4-Amino-2’,3-dimethylazobenzene diazotated |
| CAS Number | 101-89-3 |
| Empirical Formula | C₁₄H₁₄N₄O₄S |
| Molecular Weight | 334.35 g/mol |
| Color Index Number | 37210 |
| Dye Content | ≥90% |
| Form | Powder |
| Color | Orange to very dark orange / orange-brown / red |
| Solubility | Water: 5 mg/mL (clear) |
| Quality Level | 200 |
| EC Number | 202-985-4 |
| MDL Number | MFCD00011883 |
| Storage Temperature | 2–8 °C |
| Applications | Histology, cytochemistry, diagnostic assays, enzyme staining |
Ordering Information
| Pack Size | SKU |
|---|---|
| 1 g | F8761-1G |
| 5 g | F8761-5G |
| 25 g | F8761-25G |
Sigma-Aldrich™ Fast Garnet GBC sulfate salt is a high-quality diazonium dye engineered for precision in enzyme activity staining, diagnostic workflows, and histochemical visualization. Its strong dye content, reliable coupling reaction, and compatibility with established alkaline phosphatase and esterase methods make it an essential reagent for laboratories requiring accurate, high-contrast staining. Backed by Sigma-Aldrich quality and LabMart’s trusted supply chain, it provides exceptional performance for research, clinical, and industrial applications across Ghana and West Africa.
- Pack Size: 1g 5g 25g