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Sigma-Aldrich Casein from bovine milk
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suitable for substrate for protein kinase (after dephosphorylation), purified powder
Specifications:
| Application | Biochemistry | ||
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Biochemistry grade | Formula | 9000-71-9 |
Purified Phosphoprotein for Biochemical, Microbiological, and Nutritional Research
Casein from bovine milk is a purified phosphoprotein that represents approximately 80% of the total protein content in milk. It is composed mainly of four casein proteins: α-s1 casein, α-s2 casein, β-casein, and κ-casein. Due to its biochemical composition and phosphorylated structure, casein is widely used as a model protein in enzymology, protein chemistry, microbiology, nutrition, and biochemical assay development.
This purified casein powder is suitable for use in protein kinase assays, protease activity studies, protein quantification methods, microbial enzyme screening, nutritional research, and peptide generation studies. Its versatility makes it an important reagent across life science, food science, and biochemical research workflows.
Key Features
- Purified casein from bovine milk
- Phosphoprotein representing a major fraction of milk protein
- Composed of α-s1, α-s2, β-, and κ-casein proteins
- Suitable as a substrate for protein kinase assays after dephosphorylation
- Useful for protease activity studies and enzymatic digestion workflows
- Can be used as a calibrating substance in protein quantification assays
- Suitable for microbiological media supplementation
- Applicable in nutritional and peptide research studies
- Supplied as a purified powder for convenient laboratory handling
Technical Specifications
| Parameter | Details |
|---|---|
| Product Name | Casein from bovine milk |
| Brand | Sigma-Aldrich® |
| Biological Source | Bovine milk |
| Product Type | Phosphoprotein |
| Form | Purified powder |
| Main Protein Components | α-s1 casein, α-s2 casein, β-casein, κ-casein |
| Approximate Milk Protein Fraction | Approximately 80% of total milk protein |
| Melting Point | 280°C, decomposes |
| Suitability | Suitable as substrate for protein kinase after dephosphorylation |
| UniProt Accession Numbers | P02662, P02663, P02666, P02668 |
| InChI Key | BECPQYXYKAMYBN-UHFFFAOYSA-N |
| Storage Temperature | Ambient or as recommended by product documentation |
Applications
| Application Area | Typical Use |
|---|---|
| Biochemical Studies | Substrate for protein kinase assays following dephosphorylation |
| Enzymology | Study of phosphorylation, protease activity, and protein digestion |
| Protein Quantification | Calibrating substance in the biuret colorimetric method |
| Microbiology | Supplement in nutrient agar for detection of protease activity |
| Nutritional Research | Preparation of protein-enriched diets for laboratory studies |
| Peptide Research | Generation and study of bioactive peptides from partial digestion |
| Electrophoresis | Model protein for separation and protein analysis workflows |
| Immunocytochemistry | Research applications involving protein-based experimental systems |
Biochemical and Physiological Relevance
Casein is an important model protein due to its phosphorylated nature and its ability to release biologically active peptides upon digestion. Partial enzymatic digestion of casein can produce peptides such as β-casomorphins, which are of interest in biomedical and nutritional research.
Casein variants may differ in their peptide release profiles. For example, A1 and B variants of β-casein are associated with the generation of β-casomorphin-7, while A2 β-casein does not produce this peptide in the same way. This makes casein useful for comparative studies involving milk protein digestion, peptide release, and physiological activity.
Gene Information, Bovine
| Gene Symbol | Gene ID | Encoded Protein |
|---|---|---|
| CSN1S1 | 282208 | α-s1 Casein |
| CSN1S2 | 282209 | α-s2 Casein |
| CSN2 | 281099 | β-Casein |
| CSN3 | 281728 | κ-Casein |
Preparation and Handling Notes
Casein is supplied as a purified powder for reproducible laboratory use. It may be prepared for aqueous-based studies depending on the experimental protocol and required application.
Recommended handling considerations include:
- Store under recommended product conditions
- Keep container tightly closed when not in use
- Avoid contamination during weighing and preparation
- Prepare solutions or suspensions according to the required assay protocol
- Use appropriate buffers depending on the intended biochemical or microbiological application
- Follow good laboratory practice during handling
Research Use Cases
| Use Case | Description |
|---|---|
| Casein Hydrolysate Preparation | Used as a protein source for enzymatic hydrolysis and peptide generation studies |
| Biuret Protein Assay | Used as a calibrating substance in colorimetric protein quantification |
| Protease Screening | Incorporated into nutrient agar or assay systems to detect microbial protease activity |
| Nutritional Studies | Used in protein-enriched diets for experimental models such as Drosophila melanogaster |
| Kinase Assays | Used as a model substrate for studying phosphorylation activity after dephosphorylation |
Sigma-Aldrich® Casein from Bovine Milk is a purified phosphoprotein reagent suitable for a wide range of biochemical, microbiological, enzymology, protein quantification, peptide, and nutritional research applications. Its role as a model protein substrate, protein assay calibrant, microbial protease indicator, and source of bioactive peptides makes it a versatile reagent for research laboratories working in life sciences, food science, and biochemical analysis.
- Pack Size: 1kg 500g 5kg