Virplex® PNEUMOCYSTIS JIROVECII Real-Time PCR Kit, 48 Rxn

​The Virplex® PNEUMOCYSTIS JIROVECII Real-Time PCR Kit is a multiplex RT-qPCR assay for the detection and quantification of Pneumocystis jirovecii nucleic acids in human bronchoalveolar-lavage (BAL) samples.

Pneumocystis jirovecii is an opportunistic fungal pathogen responsible for Pneumocystis pneumonia (PCP), a severe respiratory infection that primarily affects immunocompromised patients. This quantitative real-time PCR kit enables accurate discrimination between colonization and active infection, supporting both diagnostic research and treatment monitoring.

Key Features

• Quantitative Detection – Includes 4 quantification standards (QS1–QS4) for estimating fungal load and distinguishing infection from colonization.
• Multiplex Design – Four targets detected in a single reaction tube per sample.
• Dual Control System – Internal control for PCR setup and inhibition monitoring + endogenous control for extraction quality.
• Rapid Results – Accurate detection and quantification in under 2 hours.
• Stable & Convenient – Lyophilized, pre-dispensed format for easy handling and reduced shipping cost.
• Instrument Compatibility – Suitable for 3-channel qPCR cyclers.
• CE-IVDR Compliant – Meets European In Vitro Diagnostic Regulation (IVDR 2017/746) requirements.

Kit Contents (48 Reactions)

• Lyophilized Master Mix (Multiplex format)
• 4 Quantification Standards (QS1–QS4)
• Internal Control
• Endogenous Control
• Positive Control
• User Manual

Technical Specifications

Applications

• Clinical Mycology Research – Detection and quantification of P. jirovecii in BAL samples.
• HIV and Immunocompromised Patient Monitoring – Assessment of PCP infection status.
• Respiratory Infection Surveillance – Differentiation between colonization and infection.
• Treatment Monitoring – Quantitative tracking of therapeutic response.
• Reference and Public Health Labs – Support for fungal infection epidemiology studies.

Ordering Information

The Virplex® PNEUMOCYSTIS JIROVECII Real-Time PCR Kit (RTPCR027-LP) provides a rapid, sensitive, and quantitative molecular solution for detecting P. jirovecii in respiratory samples. Its dual-control system, four quantification standards, and CE-IVDR compliance make it an indispensable tool for mycology research, treatment monitoring, and public health surveillance.