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Invitrogen™ Anza™ DNA End Repair Kit, 20 Rxns
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Anza™ DNA End Repair Kit is a ready-to-use reagent system that converts DNA fragments with cohesive ends into blunt-ended, 5′-phosphorylated DNA suitable for blunt-end ligation. The pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Fragment, and T4 Polynucleotide Kinase (PNK) to streamline workflows by enabling simultaneous end polishing and phosphorylation. The Anza™ 10X End Repair Buffer contains ATP and dNTPs to ensure optimal reaction conditions.
Key Features and Benefits
- Pre-mixed Enzyme Mix – Reduces pipetting and setup time
- Simultaneous Blunting and Phosphorylation – Efficient 2-in-1 functionality
- Fast Reaction Time – Complete in just 15 minutes at room temperature
- Anza-Compatible – Directly compatible with Anza™ restriction enzyme workflows
- High Reliability – Supports seamless integration into cloning protocols
Specifications
| Property | Value |
|---|---|
| Product Name | Anza™ DNA End Repair Kit |
| Catalog Number | IVGN2504 |
| Reaction Capacity | 20 reactions |
| Enzymes Included | T4 DNA Polymerase, Klenow Fragment, T4 PNK |
| Buffer Included | Anza™ 10X End Repair Buffer (with dNTPs + ATP) |
| Format | Ready-to-use liquid mix |
| Shipping Conditions | Wet or Dry Ice |
| Storage Temperature | –5°C to –30°C |
| Product Line | Invitrogen™ Anza™ |
| For Use With | Blunt-end ligation, DNA repair workflows |
| Regulatory Status | For Research Use Only |
Kit Contents
| Component | Volume |
|---|---|
| Anza DNA End Repair Mix | 100 μL |
| Anza 10X End Repair Buffer | 200 μL |
Applications
- Blunt-end cloning
- End repair of DNA fragments after restriction digestion
- Preparation of DNA for ligation into blunt-end vectors
- Polishing PCR products or sheared DNA prior to cloning
- Integration with Anza™ modular cloning systems
The Invitrogen™ Anza™ DNA End Repair Kit delivers efficient, high-performance end polishing and phosphorylation of DNA in a fast, 15-minute protocol. Ideal for cloning workflows requiring blunt-end ligation, this kit minimizes hands-on time while maximizing reliability and compatibility within the Anza™ Cloning System.