The Human Suppressors of Cytokine Signaling 1 (SOCS1) ELISA Kit is a highly sensitive immunoassay designed for the quantitative measurement of Human SOCS1 antigen in biological samples including serum, plasma, cell culture supernatants, tissue lysates, and other biological fluids.

SOCS1 is a key intracellular protein involved in regulating cytokine signaling, particularly through the Janus kinase / signal transducer and activator of transcription (JAK/STAT) pathway. By acting as a negative feedback regulator, SOCS1 plays a critical role in controlling immune responses, inflammation, and cell proliferation. Dysregulation of SOCS1 has been associated with autoimmune disorders, infectious diseases, inflammatory conditions, and cancer, making it an important target in biomedical and translational research.

This kit uses a sandwich ELISA format, where wells are pre-coated with antibodies specific to human SOCS1. Following antigen capture and washing steps, an HRP-conjugated detection antibody binds the captured antigen. The enzymatic reaction with TMB substrate generates a colorimetric signal that is proportional to the concentration of SOCS1 present in the sample and is measured at 450 nm using a microplate reader.

Key Features

• High Sensitivity: Detection limit of 12 pg/mL
• Wide Dynamic Range: 56 pg/mL – 4000 pg/mL
• High Precision
  • Intra-assay CV: <10%
  • Inter-assay CV: <12%
• Reliable Sandwich ELISA Format
• Compatible with Multiple Sample Types
  • Serum
  • Plasma
  • Urine
  • Cell culture supernatant
  • Tissue lysates
  • Other biological fluids
• Flexible Formats
  • 48-Test Kit
  • 96-Test Kit
• Ready-to-use reagents
• Colorimetric detection at 450 nm

Assay Principle (Sandwich ELISA)

1. Microplate wells are pre-coated with antibodies specific to Human SOCS1.
2. SOCS1 antigen in the sample binds to the immobilized antibody.
3. After washing, an HRP-conjugated antibody binds to the captured antigen.
4. Addition of TMB chromogenic substrate produces a blue color.
5. The reaction is stopped using an acid stop solution, turning the color yellow.
6. Absorbance is measured at 450 nm, and SOCS1 concentration is calculated from a standard curve.

Assay Workflow

Sample / Standard Addition

↓

Incubation (30 min at 37 °C)

↓

Plate Washing (5x)

↓

HRP-Conjugate Addition

↓

Incubation (30 min at 37 °C)

↓

Plate Washing (5x)

↓

TMB Substrate Reaction (15 min)

↓

Stop Solution Addition

↓

OD Measurement at 450 nm

↓

Concentration Determination from Standard Curve

Kit Components

Required Equipment (Not Included)

• ELISA Microplate Reader (450 nm)
• 37 °C Incubator
• Plate Washer (manual or automatic)
• Adjustable Micropipettes (10–1000 µL)
• Vortex mixer
• Distilled or deionized water
• Wash bottle
• Timer
• Disposable tubes
• Absorbent paper towels

Sample Preparation

Serum

Collect blood in a plain tube and allow it to clot for 10–20 minutes at room temperature. Centrifuge at 2000–3000 rpm for 20 minutes and collect the serum.

Plasma

Collect blood using EDTA or citrate anticoagulant tubes, incubate for 10–20 minutes, centrifuge at 2000–3000 rpm for 20 minutes, and collect the plasma supernatant.

Urine and Other Fluids

Collect samples in sterile tubes and centrifuge at 2000–3000 rpm for 20 minutes to obtain the clear supernatant.

Cell Culture Samples

Centrifuge culture supernatants at 2000–3000 rpm for 20 minutes and collect the clarified supernatant.

Tissue Samples

Homogenize tissues in PBS (pH 7.4) under cold conditions and centrifuge at 2000–3000 rpm for 20 minutes. Collect the supernatant for analysis.

Standard Curve Preparation

Each standard should be tested in duplicate to improve assay accuracy.

Assay Performance

Storage and Stability

• Store kit at 2–8 °C upon receipt.
• Shelf life: 6 months
• After opening:
  • Plate strips should be resealed with desiccant and stored at 2–8 °C
  • Use within 1–3 months
• Avoid repeated freeze-thaw cycles of samples.

Applications

The Human SOCS1 ELISA Kit is suitable for research applications including:

• Cytokine signaling studies
• JAK/STAT pathway research
• Immunology and inflammation research
• Cancer biology studies
• Biomarker discovery
• Drug development and therapeutic research
• Autoimmune disease investigations

The Human SOCS1 ELISA Kit (SL3093Hu) provides a reliable, sensitive, and quantitative method for detecting SOCS1 levels in a wide range of biological samples. With its high sensitivity, broad detection range, and robust sandwich ELISA design, the kit enables researchers to accurately analyze SOCS1 expression involved in cytokine signaling and immune regulation. Its standardized reagents, clear workflow, and compatibility with common laboratory equipment make it a valuable tool for immunology, oncology, and inflammation research, supporting scientists in exploring the molecular mechanisms underlying immune signaling pathways and disease progression.