Gibco™ Trypsin-EDTA (0.05%), phenol red

Gibco™ Trypsin-EDTA (0.05%) with Phenol Red is a sterile-filtered, ready-to-use cell dissociation reagent formulated for routine subculturing of adherent mammalian cells. This solution contains trypsin, a pancreatic protease derived from porcine source, combined with EDTA, a chelating agent that enhances cell detachment by disrupting calcium-dependent cell adhesion. Phenol red is included as a pH indicator for added visualization during cell processing.

This formulation provides gentle enzymatic dissociation, suitable for a wide range of adherent cell lines and primary cultures. It is manufactured under cGMP conditions at ISO 13485–certified facilities, ensuring traceability, batch-to-batch consistency, and regulatory compliance.

Key Features and Benefits

• 0.05% Trypsin with EDTA
  Provides efficient cell detachment while preserving viability and function for sensitive or strongly adherent cell types.
• Phenol Red pH Indicator
  Allows visual monitoring of buffer conditions during cell dissociation procedures.
• Sterile and Animal-Origin
  Filtered for sterility and produced from animal-derived materials (porcine pancreas) under stringent quality controls.
• Validated for Performance
  Each lot tested for sterility, pH, osmolality, mycoplasma, endotoxins, viral agents, and bioactivity.
• Global cGMP Production
  Dual-site manufacturing in USA and UK for supply continuity and full regulatory documentation.

Applications

• Routine passaging of adherent mammalian cell lines
• Enzymatic dissociation of primary cells or tissue explants
• Detachment of cells from culture vessels for reseeding
• Pre-treatment of cells for transfection or cryopreservation workflows
• Cell harvesting for assays or flow cytometry

Formulation and Components

Ordering Information

Storage and Stability

• Storage temperature: –5°C to –20°C
• Shelf life: 24 months from date of manufacture
• Shipping: Wet ice
• Packaging: Leak-proof sterile bottle

Gibco™ Trypsin-EDTA (0.05%), phenol red is a high-quality, performance-tested reagent optimized for consistent and gentle cell dissociation. It supports a wide range of applications in cell culture laboratories, ensuring safe, effective, and reproducible handling of adherent cell lines.