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Gibco™ Advanced DMEM/F-12
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With: Glucose, Non-Essential Amino Acids, Sodium pyruvate, Phenol Red
Without: L-glutamine, HEPES
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Advanced DMEM/F-12 is an optimized basal medium formulated to support mammalian cell culture with significantly reduced serum supplementation. Compared to conventional DMEM/F-12, this advanced formulation enables a 50–90% reduction in Fetal Bovine Serum (FBS) without compromising cell growth rate or morphology.
Designed for cost efficiency and improved experimental consistency, Advanced DMEM/F-12 integrates additional growth-supporting components that enhance cell viability under reduced-serum conditions, making it ideal for sensitive cell lines and serum-reduction studies.
Key Features
- Enables 50–90% reduction in FBS supplementation
- Supports growth without adaptation for many cell lines
- High glucose formulation
- Contains Non-Essential Amino Acids (NEAA)
- Includes Sodium Pyruvate
- Phenol Red pH indicator
- Sterile-filtered
- cGMP-compliant (ISO 13485)
- Suitable for reduced-serum applications
Advanced Formulation Components
Advanced DMEM/F-12 contains specialized supplements to support serum reduction:
- Ethanolamine
- Glutathione
- Ascorbic acid
- Insulin
- Transferrin
- AlbuMAX™ II lipid-rich bovine serum albumin
-
Trace elements:
- Sodium selenite
- Ammonium metavanadate
- Cupric sulfate
- Manganous chloride
Included Additives
✔ High Glucose
✔ Non-Essential Amino Acids (NEAA)
✔ Sodium Pyruvate
✔ Phenol Red
Excluded Components
✘ No L-glutamine
✘ No HEPES
Supplementation Requirement:
- 1–5% Fetal Bovine Serum (optimize per cell line)
- 4 mM L-glutamine or GlutaMAX™ supplement
Culture Conditions
- Buffer System: Sodium bicarbonate (3.7 g/L)
- CO₂ Requirement: 5–10% CO₂ incubator
- Storage: 2–8°C, protect from light
- Shipping: Ambient
- Shelf Life: 12 months from date of manufacture
Validated Cell Lines
Successfully cultured without adaptation:
- MRC-5
- SP2
- Vero
- WI-38
- Jurkat
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
| Concentration | 1X |
| Form | Liquid |
| Sterility | Sterile-filtered |
| Serum Level | Reduced Serum |
| Manufacturing Quality | cGMP-compliant (ISO 13485) |
| Classification | Animal Origin |
| Storage | 2–8°C |
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 12634010 | 500 mL |
| 12634028 | 10 × 500 mL |
Gibco™ Advanced DMEM/F-12 offers a scientifically optimized solution for laboratories seeking to reduce serum dependency while maintaining robust cell growth and morphology. By incorporating targeted supplements and trace elements, this formulation enhances culture performance under low-serum conditions, supporting improved reproducibility and cost efficiency.
- Pack Size: 1 x 500 mL 10 x 500 mL