The ONT MinION/GridION Flow Cell (DNA) – FLO-MIN114 is a high-performance sequencing consumable designed for use with Oxford Nanopore Technologies MinION and GridION sequencing devices. Each flow cell contains a sophisticated sensor array integrated with an Application-Specific Integrated Circuit (ASIC) and R10 nanopores, enabling real-time DNA sequencing with high accuracy and throughput.

The R10 nanopore architecture features a double reader-head configuration, improving base-calling resolution and enabling highly accurate sequence detection. When used with Oxford Nanopore Kit 14 sequencing chemistry, this flow cell delivers modal accuracy above 99%, making it suitable for demanding genomic and epigenomic applications.

The FLO-MIN114 flow cell supports a wide range of ONT V14 sequencing kits, including ligation, rapid sequencing, PCR-based workflows, and barcoding kits. The system enables real-time sequencing, allowing users to monitor data as it is generated and stop the experiment once sufficient data has been collected.

Key Features

R10 Nanopore Technology

• Advanced R10 nanopore design with dual reader heads
• Enhanced consensus accuracy for DNA sequencing
• Supports high-fidelity genomic and epigenomic analysis

High-Accuracy Sequencing

• Modal accuracy >99% with Kit 14 chemistry
• Real-time nanopore sequencing
• High sensitivity for complex genomic applications

Real-Time Data Streaming

• Sequencing data generated immediately during runs
• Users can monitor progress in real time
• Runs can be stopped once enough data has been generated

Long Sequencing Runtime

• Typical run time up to ~72 hours under optimal conditions
• Allows continuous sequencing or run–stop–wash–reload workflows

Flexible Sample Processing

• Compatible with multiple library preparation methods
• Supports DNA sequencing, barcoding, and targeted workflows

How It Works

1. The user prepares a DNA library using compatible ONT sequencing kits.
2. The library is loaded onto the flow cell installed in the MinION or GridION device.
3. DNA molecules pass through nanopores embedded in the flow cell membrane.
4. As DNA passes through each nanopore, changes in ionic current are detected.
5. These electrical signals are converted into DNA sequence data by base-calling software.

Sequencing results are generated in real time, allowing researchers to analyze data immediately.

Applications

The MinION/GridION Flow Cell FLO-MIN114 is widely used in:

• Whole genome sequencing
• Metagenomics
• Microbial genomics
• Structural variant analysis
• Epigenetic modification detection
• Targeted sequencing
• Clinical research and pathogen surveillance
• Environmental genomics
• Rapid outbreak detection

Compatibility

Devices

• MinION sequencing device
• GridION sequencing device

Compatible Sequencing Kits (V14 Chemistry)

• Ligation Sequencing Kit V14 (SQK-LSK114)
• Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
• Rapid Sequencing Kit V14 (SQK-RAD114)
• Rapid Barcoding Kit 24 V14 (SQK-RBK114.24)
• Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
• Native Barcoding Kit 24 V14 (SQK-NBD114.24)
• Native Barcoding Kit 96 V14 (SQK-NBD114.96)
• Ultra-Long Sequencing Kit V14 (SQK-ULK114)
• PCR Expansion (EXP-PCA001)
• PCR Barcoding Expansion 1-12 (EXP-PBC001)
• PCR Barcoding Expansion 1-96 (EXP-PBC096)
• Rapid PCR Barcoding Kit 24 V14 (SQK-RPB114.24)
• cDNA-PCR Sequencing Kit V14 (SQK-PCS114)
• cDNA-PCR Barcoding Kit V14 (SQK-PCB114.24)
• 16S Barcoding Kit 24 V14 (SQK-16S114.24)

Workflow

1. Store flow cell at 2–8 °C until use.
2. Perform a Flow Cell Check using MinKNOW software to verify nanopore availability.
3. Prepare DNA library using compatible ONT kits.
4. Load the library into the flow cell.
5. Install the flow cell light shield.
6. Start sequencing using MinKNOW software.

Sequencing data becomes available immediately as DNA molecules pass through nanopores.

Storage and Stability

• Storage temperature: 2–8 °C
• Flow cells shipped in temperature-controlled packaging
• Stable at ambient temperature for short periods during shipping

Warranty and Quality Assurance

Customers must perform a Flow Cell Check within 12 weeks of purchase. Oxford Nanopore Technologies will replace unused flow cells containing fewer than 800 active nanopores, provided:

• Storage conditions were followed
• Results are reported within two days of testing

Warranty period: 12 weeks from receipt.

Packaging and Contents

Each package contains:

• MinION/GridION Flow Cell (DNA) – FLO-MIN114
• Flow cell light shield
• Sealed protective packaging (also used for returns)

Users are encouraged to return used flow cells for responsible recycling through Oxford Nanopore Technologies.

Ordering Information

The Oxford Nanopore FLO-MIN114 MinION/GridION Flow Cell is a high-accuracy sequencing consumable designed to support advanced genomic research using nanopore sequencing technology. Equipped with R10 nanopores and optimized for Kit 14 chemistry, the flow cell delivers >99% sequencing accuracy while providing real-time data generation and flexible experimental workflows. Its compatibility with a wide range of sequencing kits and support for extended sequencing runs make it an essential component for laboratories performing genomic, metagenomic, and epigenomic analyses.