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Biologix® HEPES Buffer (1 M) – Sterile, Cell Culture Grade
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Cell Culture Buffer | Forms | Liquid |
| Product Brand | Biologix | ||
| Product Grade | Cell Culture | ||
The Biologix® HEPES Buffer (1 M) is a high-performance biological buffer widely used in cell culture and molecular biology applications. Known for its excellent buffering capacity at physiological pH, HEPES provides stable pH control in environments where CO₂-based buffering systems may be insufficient.
This sterile, ready-to-use solution is optimized for fine cell culture applications, ensuring minimal interference with biological processes while maintaining optimal cellular conditions.
Key Features & Benefits
Superior Buffering Capacity
- Maintains stable pH at 7.3 ± 0.1, ideal for physiological systems
- Effective buffering in the 10–25 mM working range
- Minimizes pH fluctuations during cell culture and experimental handling
Optimized for Cell Culture Applications
- Commonly used in fine and sensitive cell culture systems
- Supports stable conditions during manipulation outside CO₂ incubators
High Chemical & Enzymatic Stability
- Chemically stable under a wide range of experimental conditions
- Compatible with enzymatic reactions and biological systems
Low Optical Interference
- Very low absorption in visible and UV ranges
- Suitable for spectrophotometric and fluorescence-based assays
Sterile & High Purity
- Sterile and nearly endotoxin-free
- Free from phenol red indicator, ensuring no interference in assays
- Ready-to-use for aseptic operations
Applications
- Cell culture media supplementation
- Buffering of biological and biochemical reactions
- Tissue culture and cell handling outside CO₂ incubators
- Molecular biology and enzymatic assays
- Preparation of laboratory reagents and solutions
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | HEPES Buffer |
| Concentration | 1 M |
| pH | 7.3 ± 0.1 |
| Indicator | No phenol red |
| Sterility | Sterile |
| Endotoxin | Low (almost endotoxin-free) |
| Optical Properties | Low UV/visible absorption |
| Application | Cell culture, molecular biology |
| Storage Temperature | 2–8°C |
| Shelf Life | 36 months |
Ordering Information
| Catalog Number | Product Name | Concentration | Packaging |
|---|---|---|---|
| HEPES-100 | HEPES Buffer | 1 M | 100 mL / Bottle |
| HEPES-500 | HEPES Buffer | 1 M | 500 mL / Bottle |
Unique Advantages
- Provides stable pH buffering independent of CO₂ systems
- Ideal for sensitive cell culture and biochemical assays
- Low optical interference supports analytical applications
- Sterile, ready-to-use format simplifies laboratory workflows
- Reliable performance across research and applied laboratories
The Biologix® HEPES Buffer (1 M) is a reliable and essential buffering solution for laboratories requiring precise pH control in cell culture and molecular biology workflows. Its stability, purity, and ease of use make it an indispensable component for maintaining optimal biological conditions and experimental reproducibility.
- Pack Size: 100 mL 500 mL