Liofilchem® Brucella Supplement, 10 x 10 mL

Liofilchem® Brucella Supplement is a freeze-dried mixture of selective antibiotics and antifungals formulated to enhance the isolation of Brucella species by suppressing the growth of contaminating microorganisms.

When added to Brucella Agar Base (ref. 610079 / 620079), Columbia Agar Base (ref. 610013 / 620013), or Blood Agar Base N.2 (ref. 610188 / 620188), the supplement provides the selectivity necessary for recovery of Brucella spp. from milk, food, or veterinary samples while maintaining high recovery rates for slow-growing target organisms.

Key Features

• Selective supplement designed specifically for the isolation of Brucella spp.
• Broad inhibitory range—suppresses Gram-positive, Gram-negative, and fungal contaminants.
• Freeze-dried, ready-to-reconstitute format for convenient use and reproducibility.
• Each vial sufficient for 500 mL of medium.
• Enhances Brucella recovery when used with 5–10 % inactivated horse serum and 1–5 % sterile glucose.
• Validated and quality-controlled under ISO-certified manufacturing standards.

Composition per Vial

Principle of the Method

The slow growth of Brucella species requires selective agents to minimize interference from faster-growing flora.

• Polymyxin B and Nalidixic acid act on Gram-negative bacteria.
• Bacitracin and Vancomycin inhibit Gram-positive organisms.
• Cycloheximide and Nystatin suppress yeasts and molds.
  This balanced antibiotic combination creates a selective environment ideal for Brucella isolation and subsequent identification.

Preparation and Use

1. Reconstitution: Aseptically add 10 mL of a 1 : 1 mixture of methanol and sterile distilled water to one vial.
2. Dissolve completely by gentle shaking and incubate at 36 ± 1 °C for 10–15 min.
3. Add entire contents (10 mL) to 500 mL of Brucella Agar Base or other recommended basal medium cooled to 45–50 °C, already supplemented with 5–10 % inactivated horse serum and 1–5 % sterile glucose solution.
4. Mix gently and pour into Petri dishes.

Quality Control

Appearance: orange freeze-dried product 

Incubation conditions: 48 h at 36 ± 1 °C in 5–10 % CO₂

Storage and Stability

• Store at 2–8 °C in the original sealed packaging.
• Shelf life: valid until expiry date on the label (typically 4 years).
• Do not use if product shows discoloration or deterioration.

Safety and Handling

• Contains antibiotics and antifungal agents; handle with care.
• For in vitro diagnostic use only.
• Use by trained personnel in appropriately equipped microbiology laboratories.
• Refer to the Safety Data Sheet (SDS) before use.
• Dispose of materials according to local biosafety regulations.

Ordering Information

Liofilchem® Brucella Supplement provides a precise, balanced combination of selective agents that enhance the recovery of Brucella species while effectively inhibiting unwanted bacterial and fungal growth. Used with Brucella Agar Base or compatible media, it ensures reproducible isolation and differentiation of Brucella spp. from complex clinical, veterinary, and food samples—making it an essential additive for diagnostic and reference microbiology laboratories focused on Brucella detection and research.