Liofilchem® Malt Extract Broth Modified

The Liofilchem® Malt Extract Broth Modified is a basal liquid medium designed for the isolation, cultivation, and enumeration of yeasts and molds. With its nutrient-rich formulation, it supports robust fungal growth, making it ideal for environmental, pharmaceutical, and food microbiology applications.

Key Features

1. Optimized for Yeasts and Molds:
   • Provides essential nutrients and optimal pH for fungal growth while minimizing bacterial contamination.
2. Comprehensive Nutritional Profile:
   • Malt extract, peptone, and papaic digest of liver deliver the necessary carbon, proteins, and nutrients.
3. Ease of Use:
   • Simple preparation with dehydrated powder and straightforward incubation procedure.
4. Wide Application:
   • Suitable for environmental monitoring, quality control, and microbial enumeration.

Specifications

Formula

Physical Properties

Environmental and Storage

Packaging Options

Applications

1. Food and Beverage:
   • Ensures product safety by monitoring yeast and mold contamination.
2. Pharmaceuticals:
   • Verifies fungal growth in raw materials and finished products.
3. Environmental Monitoring:
   • Tracks fungal presence in air and surface samples.
4. Microbial Enumeration:
   • Suitable for fungal count in a variety of samples.

Usage Guidelines

Preparation

1. Suspend 20 g of powder in 1 liter of distilled or deionized water.
2. Heat to boiling while stirring to ensure complete dissolution.
3. Dispense into tubes or containers and autoclave at 121°C for 15 minutes.

Technique

1. Inoculate the medium with the test sample.
2. Incubate at 30 ± 2°C for 48-72 hours.
3. Observe for turbidity, indicating microbial growth.

Interpretation of Results

• Turbidity in the broth confirms the growth of yeasts or molds.

Microbial Performance

Advantages

1. Reliable Detection:
   • Supports consistent fungal growth for accurate results.
2. Nutrient-Rich:
   • Provides essential carbon and proteins to a wide variety of fungi.
3. Optimal pH:
   • Encourages fungal growth while restricting bacterial proliferation.
4. Convenient Storage:
   • Long shelf life ensures stability and availability.

Warnings and Precautions

1. Use only by trained laboratory personnel.
2. Store in a dry, dark environment to prevent contamination or deterioration.
3. Dispose of waste in compliance with local and national regulations.

References

1. Reddish (1919). Abstr. Bacteriol. 3:6.
2. Thom and Church (1926). The Aspergilli.