0
Proteintech™ BCL2 Monoclonal Antibody (4H8C6), 150 µL
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Western Blot, immunocytochemistry, Flow Cytometry, Immunohistochemistry, Immunoprecipitation (IP) | ||
| Storage Temperature | -20°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Proteintech | ||
| Product Grade | Molecular Biology | ||
The Proteintech BCL2 Monoclonal Antibody (Clone 4H8C6) is a highly validated antibody designed for the detection of BCL2 (B-cell lymphoma 2), a central regulator of apoptosis and cell survival. Targeting amino acids 1–239 of the BCL2 protein, this antibody provides high specificity and sensitivity for studying anti-apoptotic signaling pathways and cancer progression.
With extensive validation across multiple applications—including Western blot, immunohistochemistry (paraffin), immunocytochemistry, flow cytometry, and immunoprecipitation—this antibody is widely used in oncology, cell biology, and translational research. Its strong publication record and robust performance make it a reliable choice for both routine and advanced laboratory workflows.
Key Features
- Specific for BCL2 anti-apoptotic protein
- Monoclonal antibody clone 4H8C6
- Reactivity: Human, Pig (validated in additional species including mouse)
-
Validated applications:
- Western Blot (WB): 1:2,000–1:10,000
- IHC (Paraffin): 1:6,400–1:25,600
- Immunocytochemistry (ICC/IF): 1:50–1:500
- Flow Cytometry: 0.2 µg/10⁶ cells
- Immunoprecipitation (IP): 0.5–4 µg
- High-performance antibody with >200 publications supporting WB use
- Supplied as unconjugated liquid antibody for flexible assay design
- Purified using Protein A
- Stable formulation in PBS with 50% glycerol
Applications
This antibody is ideal for:
- Western blot analysis of BCL2 expression
- Immunohistochemistry (IHC) of paraffin-embedded tissues
- Immunofluorescence imaging (ICC/IF)
- Flow cytometry-based apoptosis studies
- Immunoprecipitation of BCL2 protein complexes
-
Research areas including:
- Apoptosis and mitochondrial pathways
- Cancer biology (e.g., lymphoma, prostate cancer)
- Cell survival signaling mechanisms
- Protein-protein interaction studies
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Proteintech BCL2 Monoclonal Antibody (4H8C6) |
| Catalog Number | 60178-1-Ig |
| Type | Antibody |
| Class | Monoclonal |
| Clone | 4H8C6 |
| Host / Isotype | Mouse / IgG2b |
| Species Reactivity | Human, Pig |
| Published Species | Human, Mouse, Pig |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Amount | 150 µg |
| Volume | 150 µL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Preservative | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Immunogen Information
| Parameter | Details |
|---|---|
| Immunogen | BCL2 Fusion Protein Ag3508 |
| Immunogen Region | 1–239 aa |
| Encoded By | BC027258 |
Target Information
BCL2 is a key anti-apoptotic protein that regulates cell survival by inhibiting mitochondrial pathways of apoptosis. It functions by interacting with pro-apoptotic proteins such as Bax and Bak, preventing the release of cytochrome c and subsequent activation of caspases.
Aberrant overexpression of BCL2 is strongly associated with tumorigenesis, particularly in B-cell lymphomas, where chromosomal translocation leads to its constitutive activation. Elevated BCL2 levels have also been linked to prostate cancer and other malignancies, making it a critical biomarker and therapeutic target in oncology.
Availability Note
Important: This product is currently not available in certain regions. Alternative BCL2 antibodies with similar validation and performance can be recommended upon request.
Advantages
- Broad application compatibility (WB, IHC, ICC, Flow, IP)
- High specificity and reproducibility
- Strong literature validation supporting research reliability
- Flexible unconjugated format for custom assay workflows
- Ideal for apoptosis and cancer research studies
The Proteintech BCL2 Monoclonal Antibody (4H8C6) is a robust and versatile reagent for investigating apoptosis regulation, cancer biology, and cell survival pathways. Its wide application validation, high sensitivity, and strong research backing make it a valuable tool for laboratories engaged in advanced molecular and cellular studies.