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Specifications:
| Application | Cell culture | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
The Gibco™ PBS, pH 7.2 is a high-quality phosphate-buffered saline solution designed to maintain physiological pH and osmotic balance in a wide range of cell culture and molecular biology applications. This balanced salt solution is ideal for washing cells, transporting samples, diluting reagents, and preparing biological assays, ensuring optimal cell viability and experimental consistency.
Manufactured under cGMP and ISO 13485 standards, Gibco PBS offers exceptional reliability, sterility, and batch-to-batch consistency, making it a trusted reagent for laboratories worldwide.
Key Features
- Stable pH (7.2) for controlled experimental conditions
- Without calcium and magnesium – ideal for cell dissociation workflows
- Without phenol red – eliminates interference in optical assays
- Sterile-filtered for contamination-free applications
- Maintains osmotic balance (280–320 mOsm/kg)
- Ready-to-use 1X formulation
- Manufactured under cGMP and ISO standards
Applications
This PBS solution is suitable for:
- Cell washing prior to dissociation or staining
- Transport of cells and tissue samples
- Dilution of cells for counting and analysis
- Preparation of reagents and buffers
- General laboratory washing and rinsing procedures
- Mammalian cell culture workflows
Why Use PBS Without Calcium & Magnesium
- Prevents unwanted cell adhesion and aggregation
- Facilitates efficient removal of enzymes (e.g., trypsin)
- Enhances performance in cell detachment and dissociation protocols
PBS vs DPBS
- PBS: Simpler formulation, ideal for general-purpose use
- DPBS: Contains additional components (e.g., potassium, glucose), suitable for specialized applications
Why Choose pH 7.2
- Provides slightly more acidic buffering conditions compared to pH 7.4
- Suitable for specific cell types and experimental protocols
- Offers optimized buffering capacity for certain biological systems
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Gibco™ PBS, pH 7.2 |
| Catalog Number | 20012027 |
| Solution Type | Phosphate-Buffered Saline |
| Concentration | 1X |
| pH | 7.2 |
| Volume | 500 mL |
| Additives | None (No Ca²⁺, Mg²⁺, Phenol Red, Sodium Pyruvate) |
| Osmolality | 280–320 mOsm/kg |
| Sterility | Sterile-filtered |
| Manufacturing Quality | cGMP, ISO 13485 |
| Storage | 15–30°C |
| Shelf Life | 24 months |
Advantages
- Reliable and consistent buffer performance
- Supports cell viability and integrity
- Eliminates interference in optical and biochemical assays
- Versatile use across multiple laboratory workflows
- Trusted Gibco™ quality and global reputation
The Gibco™ PBS, pH 7.2 is an essential laboratory reagent for maintaining stable physiological conditions in cell culture and molecular biology experiments. Its high purity, sterility, and flexibility make it a dependable choice for routine laboratory operations and advanced research applications.