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Specifications:
| Application | molecular biology | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
RNase-Free DNase for On-Column Genomic DNA Removal During RNA Purification
The Invitrogen™ PureLink™ DNase Set is a lyophilized, RNase-free DNase I enzyme preparation designed for efficient removal of residual DNA during RNA isolation using PureLink™ RNA kits. This set is optimized for on-column digestion, streamlining RNA workflows where complete elimination of genomic DNA is essential for downstream applications such as qRT-PCR, RNA sequencing, or transcriptome analysis.
Packaged for 50 preps, the DNase set includes a 10X reaction buffer and RNase-free water for reconstitution, and is ideal for researchers requiring DNA-free RNA preparations with maximum convenience and minimal risk of RNase contamination.
Key Features and Benefits
-
RNase-Free
Certified RNase-free, making it suitable for even the most sensitive RNA-based applications. -
On-Column Compatibility
Specifically optimized for use with PureLink™ spin column formats, allowing in-column DNA digestion without additional sample handling or transfers. -
Lyophilized for Long-Term Stability
Lyophilized format ensures extended shelf life and reliable activity upon reconstitution. -
Simple Protocol Integration
Easily incorporated into existing PureLink™ RNA purification workflows, with minimal hands-on time and no additional equipment required.
Applications
- Removal of trace genomic DNA from purified RNA
-
Preparation of RNA samples for:
- Quantitative RT-PCR (qRT-PCR)
- Transcriptome sequencing (RNA-seq)
- Microarray or hybridization assays
- cDNA synthesis workflows
- Optional add-on for enhanced purity with PureLink™ RNA kits
Kit Contents
| Component | Quantity |
|---|---|
| PureLink™ DNase I (1500 U) | Lyophilized vial |
| 10X DNase I Reaction Buffer | 1 mL |
| RNase-Free Water | 1 mL |
Technical Specifications
| Property | Details |
|---|---|
| Product Name | PureLink™ DNase Set |
| Catalog Number | 12185010 |
| Unit Size | 50 preps |
| Form | Lyophilized enzyme with liquid buffers |
| Concentration | 10X buffer included |
| Use | On-column DNase digestion |
| RNase-Free | Yes |
| Storage Conditions | Store DNase at –20°C after reconstitution; store kit at 4°C upon receipt |
| Shipping Conditions | Room temperature |
| Compatible Systems | PureLink™ RNA kits (spin column formats) |
| For Research Use | Not for use in diagnostic procedures |
The Invitrogen™ PureLink™ DNase Set is a reliable and convenient solution for eliminating residual DNA from RNA preps, ensuring the highest purity for your sensitive downstream experiments. Its on-column application, RNase-free certification, and long shelf life make it a critical component for any researcher working with RNA.