Contact us 
My Experiments 
The Invitrogen™ Novex™ AP Chemiluminescent Substrate is a ready-to-use, non-radioactive detection reagent based on CDP-Star™, designed specifically for highly sensitive detection of alkaline phosphatase (AP)-conjugated antibodies in western blotting and other blot-based immunoassays such as dot blotting, Southern blotting, and Northern blotting.
This substrate enables chemiluminescence-based detection, offering exceptional sensitivity that surpasses traditional chromogenic methods. When AP-labeled antibodies are used, the CDP-Star substrate reacts enzymatically to emit light, which can be captured using X-ray film or CCD-based imaging systems. With detection sensitivity reaching low picogram levels, this system provides researchers with high signal intensity and low background for reliable and reproducible results.
Key Features
- High Sensitivity: Detects proteins at picogram levels, offering superior performance to chromogenic substrates.
- Non-Radioactive: Safe and convenient, eliminating the need for radioactive materials.
- Ready-to-Use Format: Preformulated liquid for direct application without additional preparation steps.
- Broad Application Compatibility: Suitable for Western, Southern, and Northern blotting workflows.
- Versatile Detection Options: Compatible with both X-ray film and digital imaging platforms.
Applications
- Western blot detection of AP-labeled primary or secondary antibodies.
- Dot blotting of nucleic acids or proteins.
- Southern blot hybridization signal detection.
- Northern blotting for RNA analysis.
Product Specifications
| Parameter | Description |
|---|---|
| Product Type | Chemiluminescent Substrate |
| Substrate | CDP-Star™ |
| Enzyme Compatibility | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Quantity | 1 × 100 mL bottle |
| Detection Method | Chemiluminescence |
| Application | Blotting (Western, Southern, Northern) |
| Shipping Condition | Ambient or Wet Ice |
| Storage Condition | Store at 4°C |
The Novex™ AP Chemiluminescent Substrate (CDP-Star™) from Invitrogen™ is a reliable, high-performance solution for researchers seeking sensitive and specific detection of AP-labeled targets in blotting assays. Its ready-to-use format, broad compatibility, and excellent detection range make it ideal for a wide range of molecular biology applications.
Novex Chemiluminescent Substrates
The Invitrogen™ Novex™ AP Chemiluminescent Substrate is a ready-to-use, non-radioactive detection reagent based on CDP-Star™, designed specifically for highly sensitive detection of alkaline phosphatase (AP)-conjugated antibodies in western blotting and other blot-based immunoassays such as dot blotting, Southern blotting, and Northern blotting.
This substrate enables chemiluminescence-based detection, offering exceptional sensitivity that surpasses traditional chromogenic methods. When AP-labeled antibodies are used, the CDP-Star substrate reacts enzymatically to emit light, which can be captured using X-ray film or CCD-based imaging systems. With detection sensitivity reaching low picogram levels, this system provides researchers with high signal intensity and low background for reliable and reproducible results.
Key Features
- High Sensitivity: Detects proteins at picogram levels, offering superior performance to chromogenic substrates.
- Non-Radioactive: Safe and convenient, eliminating the need for radioactive materials.
- Ready-to-Use Format: Preformulated liquid for direct application without additional preparation steps.
- Broad Application Compatibility: Suitable for Western, Southern, and Northern blotting workflows.
- Versatile Detection Options: Compatible with both X-ray film and digital imaging platforms.
Applications
- Western blot detection of AP-labeled primary or secondary antibodies.
- Dot blotting of nucleic acids or proteins.
- Southern blot hybridization signal detection.
- Northern blotting for RNA analysis.
Product Specifications
| Parameter | Description |
|---|---|
| Product Type | Chemiluminescent Substrate |
| Substrate | CDP-Star™ |
| Enzyme Compatibility | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Quantity | 1 × 100 mL bottle |
| Detection Method | Chemiluminescence |
| Application | Blotting (Western, Southern, Northern) |
| Shipping Condition | Ambient or Wet Ice |
| Storage Condition | Store at 4°C |
The Novex™ AP Chemiluminescent Substrate (CDP-Star™) from Invitrogen™ is a reliable, high-performance solution for researchers seeking sensitive and specific detection of AP-labeled targets in blotting assays. Its ready-to-use format, broad compatibility, and excellent detection range make it ideal for a wide range of molecular biology applications.
![[T2184] Applied Biosystems™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer](/web/image/product.product/43989/image_512/%5BT2184%5D%20Applied%20Biosystems%E2%84%A2%20Luminescence%20Enhancer%20For%20Membrane%20Blotting%20Assays%2C%20Nitro-Block-II%E2%84%A2%20Enhancer?unique=96c5da2)