VIASURE®E. coli Typing Real Time PCR Detection Kit

The VIASURE E. coli Typing Real Time PCR Detection Kit is a multiplex molecular assay designed for the rapid, specific detection and differentiation of major pathogenic Escherichia coli (E. coli) pathotypes:

• Enterohemorrhagic E. coli (EHEC)
• Shiga toxin-producing E. coli (STEC)
• Enteropathogenic E. coli (EPEC)
• Enterotoxigenic E. coli (ETEC)
• Enteroinvasive E. coli (EIEC)/Shigella

This kit enables detection directly from human stool specimens through Real-Time PCR using specific primers and fluorescent probes. It includes internal controls for monitoring inhibition and ensures reliable results across high-throughput workflows. Ideal for outbreak investigation, clinical research, and public health surveillance.

Key Features & Benefits

• ✅ Multiplex Detection – Simultaneous identification of multiple E. coli pathotypes
• ✅ Ready-to-Use Format – Lyophilized 8-well strips reduce setup time and minimize pipetting error
• ✅ Internal Controls Included – Ensures result validity and detects PCR inhibition
• ✅ Room Temperature Storage – Simplifies shipping and storage logistics
• ✅ Rapid Turnaround Time – Results within hours for timely decision-making
• ✅ High Sensitivity & Specificity – Based on validated real-time PCR technology
• ✅ Positive & Negative Controls Supplied – Essential for result interpretation
• ✅ Compatible with Standard Thermocyclers – Works with most qPCR platforms

Applications

• Detection and typing of diarrheagenic E. coli in stool samples
• Outbreak surveillance of foodborne pathogens
• Differentiation of EHEC, ETEC, EPEC, EIEC, and Shigella spp.
• Research on gastrointestinal infections
• Support for public health and food safety laboratories

Kit Contents

Ordering Information

The VIASURE E. coli Typing Real Time PCR Detection Kit is a powerful molecular tool for identifying and differentiating clinically significant E. coli pathotypes in stool specimens. Its rapid results, high specificity, and user-friendly format make it ideal for clinical research, epidemiological surveillance, and outbreak response. The inclusion of internal controls, positive/negative standards, and room temperature stability supports consistent, accurate testing in both field and lab-based settings.