Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

Amicon® Ultra-2 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at 95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.

Application

• Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates.
• Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

Features and Benefits

• Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
• Concentrates 2 mL down to 15-70 μL
• 98% sample recovery
• Fast processing time: 10 to 30-minute spin times
• Engineered dead stop
• Reverse spin capability provides consistent recoveries

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, GermanyULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany