Thermo Scientific™ Pierce™ IgG Elution Buffer

Thermo Scientific™ Pierce™ IgG Elution Buffer is a ready-to-use, low-pH solution designed for efficient elution of antibodies bound to Protein A, Protein G, Protein A/G, or Protein L affinity resins. With a defined pH of 2.8, this elution buffer provides rapid and effective recovery of immunoglobulins while preserving their biological activity when neutralized immediately after elution.

This buffer is also applicable for antibody-antigen or protein-protein affinity purification systems that rely on pH-dependent binding interactions. It is manufactured under stringent quality controls, prefiltered, and optimized for compatibility with Thermo Scientific™ affinity resins and other standard IgG purification systems.

Key Features & Benefits

• Efficient IgG Elution
  Optimized for use with Protein A, G, and L affinity supports, ensuring high-yield recovery.
• Non-Denaturing Elution
  Preserves antibody structure and function when used with rapid neutralization.
• Versatile Use
  Suitable for dissociating antibodies or other proteins in pH-dependent purification workflows.
• Ready-to-Use Format
  Supplied as a 1X, prefiltered solution—no dilution or pH adjustment required.
• Consistent Performance
  Manufactured to exacting standards for reproducibility in antibody purification workflows.

Technical Specifications

Related Binding Buffers

• Protein A Binding Buffer – pH 8.0, phosphate-based, with glycerol and EDTA
• Protein G Binding Buffer – pH 5.0, with 0.02% sodium azide
• Protein A/G Binding Buffer – pH 8.0, phosphate-based, with EDTA
• Gentle Ag/Ab Elution Buffer – pH 6.6, high salt alternative for milder elution

Applications

• Affinity Purification of IgG Antibodies
  Elution from Protein A, G, A/G, or L resins after sample binding and washing.
• Protein-Protein Interaction Studies
  Reversible dissociation of interacting protein complexes using low pH.
• Antibody-Antigen Elution
  Recovery of target-specific antibodies or bound antigens for downstream analysis.
• Proteomics Sample Prep
  Elution of antibodies prior to SDS-PAGE, Western blot, or MS-based analysis.

Thermo Scientific™ Pierce™ IgG Elution Buffer (pH 2.8) offers a reliable and efficient method for eluting antibodies from common affinity resins under mild acidic conditions. Its ready-to-use liquid formulation, compatibility with Protein A, G, and L supports, and consistent performance make it a standard choice for immunoprecipitation, antibody purification, and protein complex recovery workflows.