0
Thermo Scientific™ Pierce™ Fast Blocking Buffer
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Rapid, Single-Protein Blocking Reagent for Western Blotting – Effective in Just 5 Minutes
Thermo Scientific™ Pierce™ Fast Blocking Buffer is a ready-to-use, single-protein-based blocking solution that significantly reduces western blotting workflow time. This buffer blocks PVDF and nitrocellulose membranes in just five minutes, helping minimize nonspecific binding while preserving strong, specific signal detection.
With performance comparable to traditional blocking buffers, Pierce Fast Blocking Buffer is compatible with biotin-avidin systems and common antibody formats. It is designed for seamless use with Pierce Fast Wash Buffer, which reduces total wash time to 20 minutes, making this system ideal for time-sensitive and high-throughput western blot protocols.
Key Features & Benefits
- Ultra-Fast Blocking – Achieves complete membrane blocking in just 5 minutes
- Low Background, High Signal Clarity – Minimizes nonspecific binding for crisp blot images
- Compatible with Nitrocellulose & PVDF – Versatile use across standard blotting membranes
- Biotin-Avidin System Compatible – Does not interfere with avidin-biotin-based detection
- Ready-to-Use Format – Supplied at 1X in TBST for immediate application
- Pair with Pierce Fast Wash Buffer – Reduces washing time from 90 minutes to just 20 minutes
Applications
- Traditional and high-throughput western blotting workflows
- Blocking PVDF or nitrocellulose membranes
- Use with HRP-, AP-, or fluorophore-conjugated antibodies
- Compatible with colorimetric, chemiluminescent, and fluorescent detection methods
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | Pierce™ Fast Blocking Buffer |
| Catalog Numbers | 37576 (100 mL), 37575 (500 mL) |
| Buffer Type | TBST (Tris-buffered saline with Tween 20) |
| Blocking Agent | Single purified protein with accelerant |
| Concentration | 1X (Ready to use) |
| Form | Liquid |
| For Use With | Western Blot |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Quantity | 100 mL or 500 mL |
| Storage Conditions | Store at 4°C upon receipt |
| Regulatory Use | For Research Use Only |
Thermo Scientific™ Pierce™ Fast Blocking Buffer offers unmatched speed and consistency for western blot membrane blocking. With a quick 5-minute block time and compatibility with both nitrocellulose and PVDF membranes, it is a go-to reagent for researchers who need reliable performance and time efficiency in their immunoblotting workflows.
- Pack Size: 100 mL 500 mL