Thermo Scientific™ Nystatin 85+%

Thermo Scientific™ Nystatin (≥85%, powder) is a high-potency, broad-spectrum polyene macrolide antifungal agent widely used in microbiology, pharmaceutical development, and antifungal susceptibility testing. With a minimum activity of ≥4400 IU/mg and purity of ≥85% (HPLC), this compound provides a dependable solution for research applications requiring consistent, reproducible inhibition of fungal pathogens.

Nystatin exerts its antifungal effect by binding to ergosterol, a key component of fungal cell membranes. This interaction creates ion-permeable channels that disrupt membrane integrity, causing leakage of intracellular contents and cell death. It is effective against a wide range of yeasts, molds, and fungi, including Candida albicans, Candida glabrata, Aspergillus spp., and Cryptococcus spp.

Supplied as a yellow to light brown powder, Nystatin is ideal for use in solid and liquid antifungal assays, cell culture contamination control, and drug discovery workflows. It is insoluble in water but dissolves in methanol, ethanol, and DMSO, making it suitable for organic solvent-based applications.

Key Features and Benefits

• High Antifungal Potency: ≥4400 IU/mg for reliable inhibition of pathogenic fungi
• Research-Grade Purity: ≥85% by HPLC ensures consistent performance
• Broad-Spectrum Activity: Active against Candida spp., Aspergillus spp., and other fungal organisms
• Proven Mechanism of Action: Ergosterol binding disrupts fungal membranes, leading to cell death
• Validated for Scientific Use: Trusted for pharmaceutical R&D, AST (antifungal susceptibility testing), and microbiological assays
• Stable Format: Powder with ≤6% moisture content ensures long-term storage at –20°C
• UV Validation: Absorbance at 305 nm (A ≥ 0.60) confirms biological activity
• Multiple Pack Sizes Available: 1 g, 5 g, and 25 g quantities to support lab-scale to bulk workflows

Technical Specifications

Applications

• Microbiology & Mycology Research
  Used for studying antifungal mechanisms and fungal cell membrane integrity.
• Antifungal Susceptibility Testing (AST)
  Supports EUCAST and CLSI methods for determining fungal resistance profiles.
• Pharmaceutical R&D
  Employed in antifungal drug screening, formulation stability studies, and toxicological research.
• Biopharmaceutical Production
  Applied in fungal contamination control and media supplementation for sensitive cultures.
• Clinical Microbiology Research
  Assists in fungal isolate screening and pathogenicity studies (RUO only).

Recommended Use and Handling

Preparation:

Dissolve in ethanol, methanol, or DMSO. Insoluble in water—prepare as a suspension or solvent-based stock. Avoid autoclaving or filter sterilization to preserve potency.

Working Concentrations:

• AST: As recommended by CLSI or EUCAST protocols
• Cell culture antifungal control: ~50 mg/L

Storage:

Store at –20°C in tightly sealed, light-protected containers. Prepare fresh suspensions before use as aqueous suspensions are unstable.

Ordering Information

Thermo Scientific™ Nystatin (≥85%, powder) is a reliable, research-grade antifungal compound trusted by scientists for its efficacy, purity, and stability. Whether used for fungal susceptibility testing, antifungal drug screening, or mycological studies, this high-quality polyene antibiotic supports consistent and effective results across pharmaceutical and microbiological research environments.