0
Thermo Scientific™ DreamTaq™ Green PCR Master Mix (2X)
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ DreamTaq PCR Master Mix (2X) is a ready-to-use, pre-optimized solution containing DreamTaq DNA Polymerase, a proprietary DreamTaq buffer, MgCl₂, and dNTPs. It is engineered for robust, reliable PCR amplification with minimal setup time and reduced risk of contamination. Available in colorless and green formats, the mix simplifies routine PCR workflows across molecular biology laboratories.
The DreamTaq enzyme formulation supports amplification of targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA, offering higher yields, improved sensitivity, and greater product length than conventional Taq polymerase. The green format includes a tracking dye and loading buffer, allowing direct gel loading post-PCR.
Key Features & Benefits
- ✅ Ready-to-Use Format – Minimizes pipetting steps, saving time and reducing contamination risk.
- ✅ High Amplification Efficiency – Produces higher yield PCR products than standard Taq.
- ✅ Long Product Range – Amplifies up to 6 kb from genomic DNA and 20 kb from viral templates.
- ✅ High Sensitivity – Reliable detection of low-copy targets.
- ✅ Dual Format Flexibility – Choose between colorless (for downstream applications) or green (for direct gel loading).
- ✅ Minimal Optimization Needed – Optimized buffer system ensures consistent performance.
- ✅ Stable & Convenient – Supplied with nuclease-free water for immediate use.
Applications
- Routine PCR amplification
- Genotyping and mutation screening
- High-throughput PCR workflows
- Viral DNA amplification
- DNA fragment analysis up to 6 kb
Technical Specifications
| Parameter | Details |
|---|---|
| Enzyme | DreamTaq DNA Polymerase |
| Concentration | 5 U/µL |
| Reaction Format | 2X Master Mix |
| Hot Start | No |
| Overhang Type | 3′-A |
| GC-Rich PCR Performance | Low |
| Fidelity (vs Taq) | 1X |
| dUTP Incorporation | No |
| Green Format Additives | Loading dyes and density reagents |
| Reaction Speed | Standard |
| Shipping Condition | Dry Ice |
| Storage Temperature | –20°C |
Available Formats
| Catalog Number | Format | Quantity |
|---|---|---|
| K1072 | Colorless | 20 × 1.25 mL |
| K1071 | Colorless | 4 × 1.25 mL |
| K1082 | Green | 20 × 1.25 mL |
| K1081 | Green | 4 × 1.25 mL |
The Thermo Scientific™ DreamTaq PCR Master Mix (2X) is a high-performance, user-friendly PCR solution designed for accuracy, speed, and reliability in standard DNA amplification tasks. Its superior amplification efficiency, pre-optimized formulation, and dual-format flexibility make it an essential tool for molecular biologists performing routine or high-throughput PCR. Whether for gel electrophoresis-ready products or downstream applications requiring a clean reaction mix, DreamTaq Master Mix delivers consistent, reproducible results.
- Reactions: 1000 rxn 200 rxn
- Color: Green Colorless