Contact us 
My Experiments 
Fmoc-Asp(OtBu)-OH is a high-purity, selectively protected aspartic acid derivative used in Fmoc-based solid-phase peptide synthesis (SPPS). It contains an Fmoc-protected α-amino group and a tert-butyl ester-protected β-carboxyl group, enabling controlled peptide elongation while minimizing unwanted side reactions during synthesis.
This reagent is widely used in custom peptide synthesis, pharmaceutical research, proteomics, drug discovery, enzyme-substrate studies, protein engineering, and bioorganic chemistry. Its protected structure supports efficient incorporation of aspartic acid residues into synthetic peptides with improved coupling performance and product quality.
Key Features
| Feature | Details |
|---|---|
| High Purity | ≥98.0% by HPLC for reliable and reproducible peptide synthesis |
| Fmoc Protection | Protects the α-amino group for stepwise Fmoc-SPPS workflows |
| OtBu Side-Chain Protection | Protects the β-carboxyl group of aspartic acid |
| Reduced Side Reactions | Helps minimize aspartimide formation during peptide synthesis |
| SPPS Compatible | Suitable for incorporation of aspartic acid into synthetic peptides |
| Research Applications | Ideal for peptide chemistry, proteomics, protein engineering, and drug discovery |
Chemical Properties & Identifiers
| Property | Value |
|---|---|
| Product Name | Fmoc-Asp(OtBu)-OH, ≥98.0% HPLC |
| CAS Number | 71989-14-5 |
| Empirical Formula | C₂₃H₂₅NO₆ |
| Molecular Weight | 411.45 g/mol |
| Beilstein Registry Number | 3635671 |
| EC Number | 276-251-7 |
| MDL Number | MFCD00037131 |
| PubChem Substance ID | 57651040 |
| Optical Activity | [α]²⁰/D -24 ± 2°; c = 1% in DMF |
| Form | Powder |
| Melting Point | 148–150 °C, decomposition |
| Assay | ≥98.0% by HPLC |
| Storage Temperature | 2–8 °C |
| Functional Groups | Fmoc, amine, carboxylic acid |
Applications
Fmoc-Asp(OtBu)-OH is suitable for:
- Fmoc-based solid-phase peptide synthesis
- Incorporation of aspartic acid residues into synthetic peptides
- Custom peptide synthesis and peptide modification
- Peptide-based pharmaceutical and drug discovery research
- Enzyme-substrate and inhibitor studies
- Protein engineering and structural biology research
- Bioorganic chemistry and peptidomimetic synthesis
- Workflows requiring reduced aspartimide-related side reactions
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 47618-1G-F | 1 g |
| 47618-10G-F | 10 g |
| 47618-25G-F | 25 g |
| 47618-100G-F | 100 g |
Storage & Handling
Store at 2–8 °C in a dry, tightly sealed container. Protect from moisture, oxidation, and extreme temperatures. Handle in a suitable laboratory environment using appropriate personal protective equipment, including gloves, lab coat, and safety goggles.
Research Use Statement
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Specification: Fmoc-Asp(OtBu)-OH
Fmoc-Asp(OtBu)-OH is a high-purity, selectively protected aspartic acid derivative used in Fmoc-based solid-phase peptide synthesis (SPPS). It contains an Fmoc-protected α-amino group and a tert-butyl ester-protected β-carboxyl group, enabling controlled peptide elongation while minimizing unwanted side reactions during synthesis.
This reagent is widely used in custom peptide synthesis, pharmaceutical research, proteomics, drug discovery, enzyme-substrate studies, protein engineering, and bioorganic chemistry. Its protected structure supports efficient incorporation of aspartic acid residues into synthetic peptides with improved coupling performance and product quality.
Key Features
| Feature | Details |
|---|---|
| High Purity | ≥98.0% by HPLC for reliable and reproducible peptide synthesis |
| Fmoc Protection | Protects the α-amino group for stepwise Fmoc-SPPS workflows |
| OtBu Side-Chain Protection | Protects the β-carboxyl group of aspartic acid |
| Reduced Side Reactions | Helps minimize aspartimide formation during peptide synthesis |
| SPPS Compatible | Suitable for incorporation of aspartic acid into synthetic peptides |
| Research Applications | Ideal for peptide chemistry, proteomics, protein engineering, and drug discovery |
Chemical Properties & Identifiers
| Property | Value |
|---|---|
| Product Name | Fmoc-Asp(OtBu)-OH, ≥98.0% HPLC |
| CAS Number | 71989-14-5 |
| Empirical Formula | C₂₃H₂₅NO₆ |
| Molecular Weight | 411.45 g/mol |
| Beilstein Registry Number | 3635671 |
| EC Number | 276-251-7 |
| MDL Number | MFCD00037131 |
| PubChem Substance ID | 57651040 |
| Optical Activity | [α]²⁰/D -24 ± 2°; c = 1% in DMF |
| Form | Powder |
| Melting Point | 148–150 °C, decomposition |
| Assay | ≥98.0% by HPLC |
| Storage Temperature | 2–8 °C |
| Functional Groups | Fmoc, amine, carboxylic acid |
Applications
Fmoc-Asp(OtBu)-OH is suitable for:
- Fmoc-based solid-phase peptide synthesis
- Incorporation of aspartic acid residues into synthetic peptides
- Custom peptide synthesis and peptide modification
- Peptide-based pharmaceutical and drug discovery research
- Enzyme-substrate and inhibitor studies
- Protein engineering and structural biology research
- Bioorganic chemistry and peptidomimetic synthesis
- Workflows requiring reduced aspartimide-related side reactions
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 47618-1G-F | 1 g |
| 47618-10G-F | 10 g |
| 47618-25G-F | 25 g |
| 47618-100G-F | 100 g |
Storage & Handling
Store at 2–8 °C in a dry, tightly sealed container. Protect from moisture, oxidation, and extreme temperatures. Handle in a suitable laboratory environment using appropriate personal protective equipment, including gloves, lab coat, and safety goggles.
Research Use Statement
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Specification: Fmoc-Asp(OtBu)-OH
![[160512-1G] Sigma-Aldrich™ Fmoc chloride 97%, for peptide synthesis (1g)](/web/image/product.product/38569/image_512/%5B160512-1G%5D%20Sigma-Aldrich%E2%84%A2%20Fmoc%20chloride%2097%25%2C%20for%20peptide%20synthesis%20%281g%29?unique=96c5da2)
![[47349-100G] Sigma-Aldrich™ Fmoc-Arg(Pbf)-OH, ≥98.0% (HPLC) (100g)](/web/image/product.product/38523/image_512/%5B47349-100G%5D%20Sigma-Aldrich%E2%84%A2%20Fmoc-Arg%28Pbf%29-OH%2C%20%E2%89%A598.0%25%20%28HPLC%29%20%28100g%29?unique=96c5da2)
![[47561-100G-F] Sigma-Aldrich™ Fmoc-Trp(Boc)-OH ≥97.0% (HPLC), for peptide synthesis (100g)](/web/image/product.product/38531/image_512/%5B47561-100G-F%5D%20Sigma-Aldrich%E2%84%A2%20Fmoc-Trp%28Boc%29-OH%20%20%E2%89%A597.0%25%20%28HPLC%29%2C%20for%20peptide%20synthesis%20%28100g%29?unique=96c5da2)
![[47618-100G-F] Sigma-Aldrich™ Fmoc-Asp(OtBu)-OH, ≥98.0% (HPLC), for peptide synthesis (100g)](/web/image/product.product/38528/image_512/%5B47618-100G-F%5D%20Sigma-Aldrich%E2%84%A2%20Fmoc-Asp%28OtBu%29-OH%2C%20%E2%89%A598.0%25%20%28HPLC%29%2C%20for%20peptide%20synthesis%20%28100g%29?unique=96c5da2)
![[47619-100G-F] Sigma-Aldrich Fmoc-Ser(tBu)-OH ≥98.0% (HPLC), for peptide synthesis (100g)](/web/image/product.product/38500/image_512/%5B47619-100G-F%5D%20Sigma-Aldrich%20Fmoc-Ser%28tBu%29-OH%20%E2%89%A598.0%25%20%28HPLC%29%2C%20for%20peptide%20synthesis%20%28100g%29?unique=96c5da2)
![[47625-100G] Sigma-Aldrich™ Fmoc-Glu(OtBu)-OH, ≥98.0% (HPLC), for peptide synthesis (100g)](/web/image/product.product/38503/image_512/%5B47625-100G%5D%20Sigma-Aldrich%E2%84%A2%20Fmoc-Glu%28OtBu%29-OH%2C%20%E2%89%A598.0%25%20%28HPLC%29%2C%20for%20peptide%20synthesis%20%28100g%29?unique=96c5da2)
![[47628-250G-F] Sigma-Aldrich™ Fmoc-Ile-OH, ≥98.0% (T) (250g)](/web/image/product.product/38520/image_512/%5B47628-250G-F%5D%20Sigma-Aldrich%E2%84%A2%20Fmoc-Ile-OH%2C%20%E2%89%A598.0%25%20%28T%29%20%28250g%29?unique=96c5da2)
![[47638-250G-F] Sigma-Aldrich™ Fmoc-Val-OH, ≥98.0% (HPLC) (250g)](/web/image/product.product/38516/image_512/%5B47638-250G-F%5D%20Sigma-Aldrich%E2%84%A2%20Fmoc-Val-OH%2C%20%E2%89%A598.0%25%20%28HPLC%29%20%28250g%29?unique=96c5da2)
![[8510140025] Sigma-Aldrich™ Novabiochem® Fmoc-OSu ≥99.0% (HPLC), for peptide synthesis (25g)](/web/image/product.product/38542/image_512/%5B8510140025%5D%20Sigma-Aldrich%E2%84%A2%20Novabiochem%C2%AE%20Fmoc-OSu%20%E2%89%A599.0%25%20%28HPLC%29%2C%20for%20peptide%20synthesis%20%20%2825g%29?unique=96c5da2)
![[8520080025] Sigma-Aldrich™ Novabiochem® Fmoc-Cys(Trt)-OH, ≥99.0% (HPLC) (25g)](/web/image/product.product/38505/image_512/%5B8520080025%5D%20Sigma-Aldrich%E2%84%A2%20Novabiochem%C2%AE%20Fmoc-Cys%28Trt%29-OH%2C%20%E2%89%A599.0%25%20%28HPLC%29%20%2825g%29?unique=96c5da2)
![[8520410005] Sigma-Aldrich™ Novabiochem® Fmoc-Lys(Fmoc)-OH ≥97.5% (HPLC), for peptide synthesis (5g)](/web/image/product.product/38539/image_512/%5B8520410005%5D%20Sigma-Aldrich%E2%84%A2%20Novabiochem%C2%AE%20Fmoc-Lys%28Fmoc%29-OH%20%E2%89%A597.5%25%20%28HPLC%29%2C%20for%20peptide%20synthesis%20%285g%29?unique=96c5da2)
![[8522030005] Sigma-Aldrich™ Novabiochem® Fmoc-Asn-OH, ≥97.0% (HPLC) (5g)](/web/image/product.product/38536/image_512/%5B8522030005%5D%20Sigma-Aldrich%E2%84%A2%20Novabiochem%C2%AE%20Fmoc-Asn-OH%2C%20%20%E2%89%A597.0%25%20%28HPLC%29%20%285g%29?unique=96c5da2)
![[8522070005] Sigma-Aldrich™ Novabiochem® Fmoc-Trp-OH, ≥98.0% (HPLC) (5g)](/web/image/product.product/38533/image_512/%5B8522070005%5D%20Sigma-Aldrich%E2%84%A2%20Novabiochem%C2%AE%20Fmoc-Trp-OH%2C%20%E2%89%A598.0%25%20%28HPLC%29%20%285g%29?unique=96c5da2)
![[B21030.06] Thermo Scientific™ N-Fmoc-L-valine, 98%, HPLC (5g)](/web/image/product.product/38517/image_512/%5BB21030.06%5D%20Thermo%20Scientific%E2%84%A2%20N-Fmoc-L-valine%2C%2098%25%2C%20HPLC%20%285g%29?unique=96c5da2)
![[B21079.03] Thermo Scientific™ N-Fmoc-L-serine, 97+%, ≥97.0% (HPLC, U.S. Specification) (1g)](/web/image/product.product/38513/image_512/%5BB21079.03%5D%20Thermo%20Scientific%E2%84%A2%20N-Fmoc-L-serine%2C%2097%2B%25%2C%20%E2%89%A597.0%25%20%28HPLC%2C%20U.S.%20Specification%29%20%281g%29?unique=96c5da2)
![[H27363.06] Thermo Scientific™ N-Fmoc-S-trityl-L-cysteine, 95%, HPLC (5g)](/web/image/product.product/38496/image_512/%5BH27363.06%5D%20Thermo%20Scientific%E2%84%A2%20N-Fmoc-S-trityl-L-cysteine%2C%2095%25%2C%20HPLC%20%285g%29?unique=96c5da2)
![[H62936-22] Thermo Scientific™ N-Fmoc-L-glutamic acid, 95%, HPLC (100g)](/web/image/product.product/38495/image_512/%5BH62936-22%5D%20Thermo%20Scientific%E2%84%A2%20N-Fmoc-L-glutamic%20acid%2C%2095%25%2C%20HPLC%20%28100g%29?unique=96c5da2)
规格
| Pack Size | 100g, 1g, 10g, 25g |