Sigma-Aldrich Tris-EDTA buffer solution, BioUltra, for molecular biology, pH 8.0 (copy)

The Sigma-Aldrich Lysozyme from Chicken Egg White is a highly effective enzyme used for breaking down bacterial cell walls, genomic DNA extraction, and other biotechnological applications. With a specific activity of ≥39,000 units/mg protein, this enzyme is reliable for a wide range of cell lysis and analytical procedures.

Key Features:

1. High Activity:
   • Specific activity of ≥39,000 units/mg protein, ensuring efficient cell lysis and enzymatic activity.
2. Broad pH Range:
   • Active between pH 6.0 to 9.0, with optimal performance at pH 6.2 for a wide range of ionic strengths.
3. Versatile Applications:
   • Effective for Gram-positive bacterial lysis due to the high peptidoglycan content in their cell walls.
   • Can hydrolyze Gram-negative bacterial walls in the presence of EDTA, which disrupts the outer membrane.
4. Stable and Reliable:
   • Available as a powder or granules for ease of handling.
   • Shipped on wet ice to maintain enzyme stability during transport.
5. Biological Source:
   • Derived from chicken egg white, offering a reliable and high-quality enzyme for research and industrial applications.

Applications:

1. Genomic DNA Extraction:
   • Efficiently breaks down bacterial cell walls to facilitate the isolation of intact genomic DNA for molecular biology studies.
2. Cell Lysis for Protein Analysis:
   • Prepares bacterial spheroplasts for downstream applications, including protein analysis and structural studies.
3. Mass Spectrometry:
   • Used as an external standard in MALDI-TOF (Matrix-Assisted Laser Desorption Ionization-Time of Flight) mass analysis.
4. Biochemical Research:
   • Investigates peptidoglycan structures in bacterial cell walls.
   • Studies enzymatic activity under various pH and ionic conditions.

Biochemical/Physiological Actions:

• Hydrolyzes β(1→4) linkages in bacterial peptidoglycan between N-acetylmuramic acid and N-acetyl-D-glucosamine.
• Also hydrolyzes N-acetyl-D-glucosamine residues in chitodextrin.
• Particularly effective against Gram-positive bacteria due to their high peptidoglycan content.
• Gram-negative bacteria are susceptible when treated with EDTA to disrupt their outer membrane.

Unit Definition:

• One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute, measured by turbidimetric detection at 600 nm at pH 6.2 and 25 °C.

Specifications:

Available Pack Sizes:

Storage and Shipping:

• Shipped on wet ice to ensure stability.
• Store at recommended conditions to maintain enzymatic activity.

Advantages:

1. High Purity and Activity:
   • Ensures effective and consistent results in cell lysis and enzymatic applications.
2. Versatility:
   • Suitable for a wide range of research applications, including DNA extraction, protein analysis, and bacterial studies.
3. Convenience:
   • Available in multiple packaging sizes to meet various research and industrial needs.

The Sigma-Aldrich Lysozyme from Chicken Egg White is a versatile and essential tool for researchers and biotechnologists, providing efficient bacterial lysis and enzymatic activity across diverse applications. Its high activity and broad pH range make it ideal for molecular biology, microbiology, and analytical chemistry workflows.