Sigma-Aldrich Giemsa′s azur eosin methylene blue solution for microscopy

Ready-to-Use Giemsa Staining Solution for Blood, Bone Marrow, and Histological Applications

Sigma-Aldrich Giemsa Azure Eosin Methylene Blue Solution is a high-quality, ready-to-use staining reagent for microscopy, commonly used in hematology, histology, and clinical cytology. This stain is widely applied for blood and bone marrow smears, paraffin sections, and clinical-cytological specimens, offering superior morphological differentiation compared to H&E staining.

Giemsa staining provides distinct nuclear and cytoplasmic staining, with chromatin structures appearing in various blue shades and acidophilic components in red shades, making it a versatile stain for diagnostic and research applications.

This product is CE-marked and IVD registered, ensuring compliance with clinical and laboratory standards.

Key Features & Benefits

✅ Ready-to-Use Solution – No additional preparation required

✅ High-Quality Microscopy Stain – Suitable for histology, hematology, and cytology

✅ Enhanced Cellular Morphology Differentiation – Superior to H&E staining

✅ CE-Marked & IVD Registered – Ensures clinical compliance and reliability

✅ Versatile Applications – Compatible with Pappenheim (MGG) staining when combined with May-Grünwald’s stain

✅ Stains Nuclei Red & Cytoplasm in Varied Shades – Based on the interaction between Azure B, Eosin Y, and DNA

✅ 500 mL Bottle Covers 3,500–5,000 Stainings – Cost-effective for high-throughput labs

✅ Compatible with Various Specimen Types – Stains blood smears, bone marrow, and paraffin sections

Applications

🔬 Hematological Staining – Used for blood and bone marrow smears

🦠 Histology & Clinical Cytology – Applied in paraffin sections and cytological specimens

🧬 Microscopy & Cellular Morphology Analysis – Enhances differentiation of cell components

⚗️ Diagnostic & Research Applications – Used in pathology, hematology, and biomedical studies

🛠 Pappenheim (MGG) Staining – Frequently combined with May-Grünwald’s stain for comprehensive overview staining

Ordering Information

Staining Mechanism & Color Interpretation

• DNA-Rich Nuclei → Red shades due to Eosin Y - Azure B-DNA complex interaction
• Cytoplasm & Acidophilic Components → Various red tones
• Chromatin Structures → Blue shades, influenced by pretreatment and fixation
• Staining Intensity & Hue → Depends on Azure B content, Azure B:Eosin Y ratio, fixation, staining time, and pH levels

Usage Guidelines

• Apply directly to prepared slides for staining of blood smears, bone marrow, or paraffin sections
• For hematology, use in combination with May-Grünwald’s solution for Pappenheim staining (MGG)
• Ensure proper fixation of specimens for optimal staining results
• Use appropriate buffer solutions to maintain pH consistency and avoid staining variability
• Follow recommended incubation times to achieve desired intensity and contrast

Regulatory & Safety Information

⚠️ For In Vitro Diagnostic Use (IVD) – CE-marked and compliant with laboratory regulations

• Handle with appropriate laboratory safety precautions
• Store in a cool, dry place away from direct sunlight
• Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions

Why Choose Sigma-Aldrich Giemsa Staining Solution?

🔹 Superior Cellular Differentiation – Enhances morphological contrast better than H&E staining

🔹 Reliable & High-Quality Staining – Trusted for hematology, cytology, and histology

🔹 Easy-to-Use, Pre-Mixed Solution – Eliminates preparation errors & ensures consistency

🔹 Compatible with Pappenheim (MGG) Staining – Ideal for hematological applications

🔹 CE-Certified for Clinical & Research Use – Ensures laboratory compliance and safety

For bulk orders, technical support, or additional information, contact LabMart Limited today.