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Thermo Scientific™ Middlebrook 7H9 Broth (Dehydrated)
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Isolate and cultivate Mycobacterium spp.
Specifications:
| Application | Clinical microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Remel | ||
| Product Grade | Microbiology grade | ||
Enrichment Liquid Medium for the Cultivation of Mycobacterium spp.
Middlebrook 7H9 Broth, developed by Middlebrook and Cohn in 1958, is a standard liquid medium widely used for the isolation, cultivation, and maintenance of Mycobacterium species, including Mycobacterium tuberculosis. Supplied in a dehydrated powder format, this Thermo Scientific™ Remel™ formulation supports luxuriant mycobacterial growth, especially when supplemented with ADC enrichment. It is well-suited for use in biochemical testing, culture transfers, and diagnostic microbiology workflows.
Key Features
- Supports Mycobacterial Growth: Promotes robust and reproducible growth of Mycobacterium spp., including slow-growing and fastidious strains
- Compatible with Enrichment: Designed for use with ADC enrichment to provide catalase, dextrose, and albumin for optimal growth
- Enhanced Biochemical Profiling: Suitable for transferring cultures to biochemical testing media
- Dehydrated Format: Allows flexible media preparation in high-throughput or small-volume labs
- Nutrient-Rich Base: Includes ammonium sulfate (nitrogen source), biotin, and pyridoxine to stimulate enzymatic and metabolic activity
- Qualitative Applications: Used in mycobacteriological diagnostics and research
Applications
- Clinical Microbiology: Cultivation of M. tuberculosis from respiratory, tissue, or fluid specimens
- Public Health Laboratories: TB surveillance, drug susceptibility, and outbreak investigations
- Pharmaceutical Microbiology: Contamination screening and mycobacteria recovery
- Mycobacterial Research: Laboratory maintenance of pure Mycobacterium cultures for molecular or biochemical studies
Specifications Table
| Parameter | Details |
|---|---|
| Product Name | Middlebrook 7H9 Broth (Dehydrated) |
| Catalog Numbers | R454012 (500 g) |
| Product Type | Culture media – broth (powder) |
| Format | Dehydrated powder |
| Enrichment Required | ADC supplement recommended |
| Target Organisms | Mycobacterium spp. |
| Nutrients | Inorganic salts, ammonium sulfate, biotin, pyridoxine |
| Intended Use | Qualitative cultivation of mycobacteria |
| Storage Conditions | Store in a cool, dry place |
| Compliance | For laboratory/research use only (non-IVD) |
Ordering Information
| Catalog Number | Pack Size |
|---|---|
| R454012 | 500 g |
Thermo Scientific™ Remel™ Middlebrook 7H9 Broth is a trusted liquid medium for the reliable cultivation of Mycobacterium species. Its flexible dehydrated format, compatibility with ADC enrichment, and optimized formulation make it ideal for use in clinical, industrial, and research laboratories handling mycobacterial cultures.