Quantabio qScript cDNA Synthesis Kit

Features & Benefits

• Economical 2-component kit ideally suited for high throughput expression studies
• Optimized, double pre-primed master mix component ensures equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
• Broad linear dynamic detection range with limiting (10pg) or plentiful (1ug) samples of total RNA

qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.

• Supermix format ideal for high throughput applications
• Unbiased representation gives confidence that RNA is represented accurately in the resulting cDNA
• Broad dynamic range ensures data from precious samples is more reliably attained
• Choice of priming methods to suit individual needs

qScript™ cDNA SuperMix is an easy to use solution for two-step RT-PCR. This one-tube supermix contains all components for cDNA synthesis including buffer, dNTPs, MgCl₂, primers, RNase inhibitor, qScript™ Reverse Transcriptase and stabilisers. Only RNA needs to be added. Complete cDNA synthesis is achieved in 40 minutes. Consistency and reproducibility are achieved over a broad dynamic range regardless of RNA input. In addition, qScript™ cDNA SuperMix offers excellent sensitivity and accurate representation of low abundance genes. The supermix format eliminates multiple component additions providing exceptional reproducibility and precision. qScript™ cDNA SuperMix is available in both single tube or 96-well format.

qScript™ cDNA Synthesis Kit is a sensitive and easy to use kit for RNA quantification using two-step RT-PCR. This kit contains an optimised blend of random and oligo (dT) primers for robust, consistent and unbiased first-strand synthesis over a broad range of RNA template concentrations. The qScript™ Reaction Mix (5X) contains all required reagents for cDNA synthesis, except for qScript™ Reverse Transcriptase and RNA. qScript™ Reverse Transcriptase is provided in a separate tube. This kit is intended for use by researchers using Bio-Rad iScript™ cDNA Synthesis Kit for ongoing studies or established Standard Operating Procedures that cannot readily be switched to qScript™ cDNA SuperMix.

qScript™ Flex cDNA Kit is an easy to use format for first-strand cDNA synthesis that supports multiple RNA priming strategies. The qScript™ Flex reaction mix (5X) is optimised for use with oligo dT, random or gene specific primers in any combination. A proprietary cDNA-priming enhancer that improves sensitivity is provided pre-mixed with random and oligo dT primers, and in a separate tube for use with gene specific primers (user provided). qScript™ Reverse Transcriptase is provided in a separate tube