Invitrogen™ Qubit™ Endotoxin Detection Assay Kit, 80 Rxn

The Invitrogen™ Qubit™ Endotoxin Detection Assay Kit is a highly sensitive and convenient fluorescent endpoint assay designed for quantifying endotoxin levels in protein, peptide, antibody, and nucleic acid samples. Compatible with the Qubit Flex Fluorometer, this kit uses amebocyte lysates to provide accurate and reliable measurements of endotoxin concentrations, helping ensure the purity and safety of samples used in research.

Key Features

1. High Sensitivity:
   • Detects endotoxin levels from 0.01 EU/mL to 10.0 EU/mL.
   • Offers a broad dynamic range for diverse sample types.
2. Versatility:
   • Suitable for various sample types, including:
     • DNA, RNA, plasmids
     • Proteins and antibodies
   • Assesses endotoxin contamination across multiple applications.
3. Ease of Use:
   • Designed for seamless integration with the Qubit Flex Fluorometer:
     • Automatic calculations reduce the potential for errors.
     • Streamlined workflow enhances efficiency.
4. Accurate Quantification:
   • Includes a validated endotoxin standard derived from E. coli strain O111:B4 for precise measurement.
   • Optimized to evaluate the effectiveness of endotoxin removal methods.
5. Fluorescence-Based Detection:
   • Generates a strong fluorescent signal proportional to the endotoxin concentration.
   • Reliable endpoint assay ensures reproducibility and sensitivity.

Applications

• Endotoxin Quantification:
  • Evaluate endotoxin contamination in samples prior to use in sensitive applications.
• Quality Control:
  • Assess the efficacy of endotoxin removal methods.
• Research Applications:
  • Protect tissue culture cells and animal models from endotoxin-induced shock, inflammation, or sepsis.

Mechanism of Action

1. Amebocyte Lysate Reaction:
   • Endotoxin lipopolysaccharides (LPS) activate a series of enzymatic reactions in the lysate.
   • This leads to the activation of Factor C, Factor B, and the pro-clotting enzyme.
2. Signal Generation:
   • The activated enzyme cleaves a substrate, producing a strong fluorescent signal.
3. Quantification:
   • The signal is directly proportional to the endotoxin concentration and measured on the Qubit Flex Fluorometer.

Specifications

Kit Contents

• Reagents sufficient for 80 reactions.
• Includes all necessary components for fluorescence-based endotoxin quantification.

Notes

1. Equipment Compatibility:
   • Designed for use with the Qubit Flex Fluorometer.
   • Not compatible with Qubit 4 Fluorometers.
2. Verification Recommended:
   • Use the Qubit Flex System Verification Assay Kit before the first assay run to ensure accuracy and consistency.
3. For Research Use Only:
   • Not intended for diagnostic procedures.

Advantages

• Accuracy:
  • High sensitivity ensures detection of even low endotoxin levels.
• Efficiency:
  • Fluorescence-based endpoint assay simplifies workflows.
• Flexibility:
  • Suitable for a wide range of biological samples and applications.
• Safety:
  • Protects cells and animal models from endotoxin-related complications.

The Invitrogen™ Qubit™ Endotoxin Detection Assay Kit is an essential tool for researchers aiming to ensure the safety and quality of biological samples in sensitive applications. Its high sensitivity, ease of use, and compatibility with the Qubit Flex Fluorometer make it a reliable choice for endotoxin quantification.