Promega GoTaq® Master Mix

All-in-One Optimized PCR Master Mix—Just Add Template, Primers and Water

• Directly substitute into your current PCR conditions
• Use the green master mix for direct-to-gel analysis after amplification
• Use the colorless master mix for post-amplification analysis by fluorescence or absorbance without prior DNA purification

Set Up PCR in Less than a Minute with Convenient Master Mixes

GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq DNA polymerase.

For ultimate in convenience, choose GoTaq® Colorless Master Mix or GoTaq® Green Master Mix and directly substitute in your current PCR application—no need to change cycling parameters.

Both Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp).

GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels.

Reactions can be set up in less than a minute; simply add your template, water and primers and go!

GoTaq® Green and Colorless Master Mix reaction size is based on a 50µl reaction volume.