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Specifications:
| Application | Biochemical Assays | ||
| Storage Temperature | -20°C | ||
| Product Type | Biochemical Reagent | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | Formula | CH₃CO₂C₁₀H₇ |
High-purity esterase substrate for biochemical assays, zymography, and enzymatic activity detection
Sigma-Aldrich™ 2-Naphthyl Acetate (β-Naphthyl Acetate) is a high-quality ester substrate widely used in esterase assays, enzyme activity visualization, lipase characterization, and zymography applications. With a purity level ensuring <0.2% free naphthol, this reagent delivers precise, sensitive detection of non-specific esterase and β-esterase activities in biological samples.
This compound is supplied as a stable crystalline powder and is commonly employed in SDS-PAGE gel staining protocols and biochemical assays in enzymology, proteomics, and developmental biology.
Key Features & Benefits
High-Purity Enzyme Substrate
- Ideal for detecting esterase and lipase activities
- Low impurities (<0.2% free naphthol) ensures clean analytical backgrounds
- Excellent consistency for research and diagnostic workflows
Versatile Biochemical Applications
Used as a substrate in:
- Zymography / SDS-PAGE esterase staining
- Lipase profiling in larvae and adult specimens
- Non-specific esterase assays
- β-esterase enzyme quantification
- Enzyme localization and activity visualization
Optimized for Clear Background Staining
- Dissolves effectively in ethanol or acetone
- Ethanol-based solutions provide very light or no background, eliminating the need for destaining
- Produces sharp, well-defined enzyme bands in gels
Chemical & Physical Information
| Parameter | Details |
|---|---|
| Chemical Name | 2-Naphthyl acetate / β-Naphthyl acetate |
| Linear Formula | CH₃CO₂C₁₀H₇ |
| CAS Number | 1523-11-1 |
| Molecular Weight | 186.21 g/mol |
| EC Number | 216-194-7 |
| MDL Number | MFCD00004060 |
| Form | Powder |
| Impurities | <0.2% free naphthol |
| Melting Point | 67–70 °C (lit.) |
| Solubility | 50 mg/mL (ethanol) |
| Storage Temperature | −20 °C |
| NACRES Code | NA.32 |
Applications
1. Enzyme Activity Detection
- Sensitive substrate for lipases, esterases, and hydrolases
- Quantification of non-specific esterase activity in insects, tissues, and cell extracts
2. Zymogram & SDS-PAGE Analysis
- Used in gel-based assays to visualize enzyme activity directly
- Enables identification of specific esterase-producing protein bands
3. Biochemical & Physiological Studies
- Supports characterization of metabolic pathways
- Used in developmental biology and entomology research
Ordering Information
| Pack Size | SKU |
|---|---|
| 5 g | N6875-5G |
Sigma-Aldrich™ 2-Naphthyl Acetate is a reliable, high-purity substrate for esterase and lipase assays, zymography, biochemical analysis, and enzyme characterization. Its sharp activity visualization, low background staining, and convenient solubility make it an essential reagent for enzymology and proteomics workflows.