0
Specifications:
| Application | Immunohistochemistry, Immunofluorescence, Magnetic Affinity Separation, Fluorescence-Based Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Miltenyi Biotec | ||
| Product Grade | Molecular Biology | ||
The Anti-APC MicroBeads are designed for highly specific and efficient magnetic labeling and separation of cells stained with APC-conjugated primary antibodies, peptides, or ligands. These monoclonal anti-APC antibody-coated MicroBeads enable positive selection or depletion of fluorescently labeled target cells using magnetic cell sorting (MACS®) technology.
With high sensitivity and specificity, Anti-APC MicroBeads allow for precise isolation of rare or weakly expressed cell populations. They are compatible with various MACS® separation columns, making them a versatile tool for immunology, flow cytometry, and antigen-specific T-cell enrichment applications.
Key Features & Benefits
1. High-Specificity Magnetic Labeling for APC-Tagged Cells
- Anti-APC monoclonal antibody-coated MicroBeads efficiently bind APC-conjugated primary antibodies.
- Enables positive selection or depletion of fluorescently labeled cells.
- Ensures minimal background staining and high-purity cell separation.
2. Enhanced Sensitivity for Low-Expression Markers
- Ideal for isolating cells with weakly expressed markers, where APC labeling provides a strong fluorescence signal.
- Facilitates detection and enrichment of antigen-specific T cells using APC-conjugated peptide/MHC-tetramers.
- Allows flow cytometric analysis without further staining.
3. Compatible with a Range of Magnetic Cell Separation Columns
- For positive selection: Compatible with MS, LS, XS, or autoMACS® Columns.
- For depletion: Works with LD, D, or autoMACS® Columns.
4. Ready-to-Use Lyophilized Format Available
- Available in liquid suspension or lyophilized format for extended storage.
- Flexible scalability for processing different sample sizes and cell numbers.
Ordering Information
| Catalog Number | Description | Capacity |
|---|---|---|
| 130-090-855 | Anti-APC MicroBeads | For 1×10⁹ total cells |
| 130-097-143 | Anti-APC MicroBeads (Lyophilized) | For 1×10⁹ total cells |
Applications & Use Cases
1. Cell Separation & Enrichment for Immunology Research
- Isolation of APC-labeled immune cells, including T cells, B cells, and dendritic cells.
- Enrichment of antigen-specific T cells using APC-labeled tetramers for T-cell receptor (TCR) studies.
2. Flow Cytometry & Fluorescence-Activated Cell Sorting (FACS)
- APC-labeled cell populations can be directly analyzed by flow cytometry after magnetic separation.
- Enhances sensitivity for weakly expressed markers, improving downstream detection.
3. Positive Selection & Depletion of APC-Tagged Cells
- Enables high-purity isolation of APC-positive cells for functional assays.
- Depletes APC-labeled unwanted cells from mixed populations for enriched downstream analysis.
Column Compatibility for Magnetic Separation
| Separation Type | Compatible MACS® Columns |
|---|---|
| Positive Selection | MS, LS, XS, autoMACS® |
| Depletion | LD, D, autoMACS® |
The Anti-APC MicroBeads offer a highly specific, magnetic separation solution for APC-labeled cells, enabling precise enrichment, depletion, and downstream analysis in immunology and flow cytometry applications. With compatibility across multiple MACS® columns and lyophilized availability, these beads provide a versatile and efficient tool for cell sorting and antigen-specific T-cell studies.
- Size: MicroBeads for 1×109 total cells Lyophilized MicroBeads for 1×109 total cells