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Long-Range PCR: GoTaq® Long PCR Master Mix
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Specifications:
| Application | qPCR | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Promega | ||
| Product Grade | Molecular Biology | ||
GoTaq® Long PCR Master Mix is a specialized PCR solution optimized for the amplification of long DNA amplicons, up to 30 kb from human genomic DNA and 40 kb from lambda DNA. It combines the robust performance of GoTaq® Hot Start Polymerase with a proprietary thermostable proofreading enzyme, delivering enhanced fidelity, processivity, and yield.
Designed to support mutational analysis, cloning, and sequencing, this master mix provides convenience with its hot-start formulation for room temperature reaction setup, and is ideal for demanding PCR workflows requiring long-range amplification with high specificity.
Key Features and Benefits
- Amplifies long DNA targets: Up to 30 kb (gDNA), 40 kb (lambda DNA)
- Enzyme blend: GoTaq® Hot Start + proofreading polymerase for high fidelity
- Hot-start formulation: Minimizes non-specific amplification and primer dimers
- Highly processive: Supports consistent elongation across long templates
- Pre-mixed 2X master mix: Simplifies PCR setup and reduces pipetting errors
- Includes control primer pair and template for system validation and QC
- Ideal for gene cloning, sequencing, mutational analysis, and structural genomics
Applications
- Long-range PCR up to 40 kb
- Gene cloning of full-length transcripts
- Genomic region analysis and mutagenesis
- DNA sequencing preparation (Sanger or NGS)
- High-complexity templates with GC-rich regions
Technical Specifications
| Attribute | Description |
|---|---|
| Format | 2X Master Mix with enzyme blend |
| Polymerase Components | GoTaq® Hot Start + proofreading polymerase |
| Fidelity | Higher than standard Taq due to proofreading enzyme |
| Amplicon Length Capability | Up to 40 kb (lambda), 30 kb (human gDNA) |
| Enzyme Functionality | 5'→3' polymerase, 3'→5' proofreading |
| Hot Start Activation | Yes (chemical modification) |
| Included Controls | Primer pair and human gDNA for validation |
| Storage | –20 °C |
| Packaging | Supplied with detailed protocol and QC guide |
| Intended Use | Research Use Only (RUO) |
GoTaq® Long PCR Master Mix (M4021) is a high-performance, hot-start enzyme mix specifically designed for accurate, efficient amplification of long DNA fragments. Whether amplifying genomic regions for sequencing or cloning full-length genes, this mix offers the reliability, convenience, and high yield demanded by advanced molecular biology workflows.