Liofilchem® Polymyxin B PB 0.064-1024 10 MIC test

The Liofilchem™ MTS™ Polymyxin B MIC Test Strips are precision tools for determining the Minimum Inhibitory Concentration (MIC) of Polymyxin B against bacterial pathogens. These strips provide a reliable and efficient gradient diffusion method for antimicrobial susceptibility testing, offering a wide range of concentrations (0.064–1024 µg/mL) to assess bacterial susceptibility to Polymyxin B.

Key Features

1. Wide MIC Range:
   • Covers a broad concentration range (0.064–1024 µg/mL), allowing testing of organisms with varying susceptibility levels, including multidrug-resistant strains.
2. High Sensitivity and Accuracy:
   • Enables precise MIC determination, critical for guiding treatment decisions, especially for infections caused by Gram-negative pathogens.
3. Gradient Diffusion Technology:
   • Provides a continuous gradient of Polymyxin B concentrations on a single strip, ensuring accurate identification of the MIC value.
4. Ready-to-Use:
   • Strips are pre-calibrated and packaged for immediate application in clinical and research laboratories.
5. Applications for Multidrug-Resistant Organisms:
   • Effective for Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii, particularly in cases of suspected resistance to polymyxins.

Applications

• Clinical Microbiology:
  • Assess susceptibility of multidrug-resistant Gram-negative bacteria.
  • Determine MIC values for guiding therapeutic decisions in critical care settings.
• Research:
  • Study resistance mechanisms in bacteria, including polymyxin resistance.
  • Explore new therapeutic strategies and combination therapies involving Polymyxin B.
• Veterinary and Environmental Microbiology:
  • Monitor antimicrobial susceptibility in veterinary pathogens.
  • Test environmental samples for resistant bacterial strains.

Testing Procedure

1. Preparation:
   • Prepare a bacterial suspension adjusted to 0.5 McFarland turbidity.
   • Spread the suspension evenly onto a Mueller-Hinton agar plate.
2. Application:
   • Place the Polymyxin B MTS™ strip onto the inoculated plate using sterile forceps.
3. Incubation:
   • Incubate at 35–37°C for 16–20 hours under aerobic conditions.
4. Results Interpretation:
   • Read the MIC value where the edge of the bacterial inhibition zone intersects the strip.

Storage and Stability

• Long-Term Storage: Store at -20°C for extended stability.
• Short-Term Storage: Store at 2–8°C for immediate use.
• Keep the strips in a sealed, moisture-free environment to maintain potency.

Advantages

1. Reliable Results:
   • Ensures consistent and reproducible MIC values, meeting clinical and research requirements.
2. Enhanced Testing Range:
   • Wide MIC range allows for comprehensive testing of susceptible and resistant strains.
3. Ease of Use:
   • Simple setup integrates seamlessly with standard laboratory workflows.

Packaging

• Supplied in individually sealed packs for sterility and long shelf life.
• Available in various quantities to suit laboratory needs.

Why Choose Liofilchem™ MTS™ Polymyxin B MIC Test Strips?

The Liofilchem™ MTS™ Polymyxin B MIC Test Strips provide an accurate, efficient, and reliable method for assessing bacterial susceptibility to Polymyxin B. With their wide MIC range and proven reliability, these strips are indispensable tools for clinical, research, and veterinary microbiology laboratories.